Share your poster with the scientific world!
Welcome to ePosters.net, the open-access journal where you can share your scientific or medical poster with the world.
After you present your poster at a conference or meeting you why not upload it here at ePosters.net? ePosters.net incorporates an advanced new search engine which scans all the words within the pdf allowing relevant posters to be quickly and accurately identified by researchers.
Benefits of submitting your poster:
- Easy and free - both submission and access are completely free of charge*
- Citation - you can cite your poster by its unique reference number and date on your résumé
- Worldwide exposure – access to your work is no longer restricted to just meeting attendees
- Easy to find - your poster can always be found using keywords or its unique reference number
- Increase collaboration - readers can contact you to discuss your work via anonymous email
- No loss of copyright – we do not require you to assign us any copyright on your material
- Reduce admin – interested viewers can print out your poster locally on their own printer
* For commercial companies there will be a small administration charge for each poster published. Quantity and sponsorship opportunities are also available. Please contact us for more information
ePosters.net is now accepting submissions
We want the service provided by ePosters.net to be always available free of charge, thereby the ensuring widest possible sharing of new scientific work across the globe.
Of course the vast potential audience for this facility should be of interest to sponsoring companies who wish to assist in the support of this service to the scientific community. For example :
- Companies may wish to secure more exposure for the work (and investment) already expended in the preparation of their own posters. This can be facilitated by selecting the company name from the Sponsors drop down box on the home page.
- Conference organizers may wish to promote their meetings – over and above the free reciprocal arrangements we offer – by means of a banner ad either on the top of the home page or at the side of internal pages.
If you want to learn more about how your company could benefit from the opportunities available on ePosters.net please email us for more information.
A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
The ability to express and extract fully functional proteins in sufficient quantities from bacterial cultures is a prerequisite for many projects in which recombinant proteins are required for structural studies, functional characterization, as assay components or for other applications. However, low yield, poor solubility and lack of functionality are often associated with, what should be, a routine procedure.
Magneto-Hydrodynamic Focussing for Point of Care Applications
Magnetic bead handling is a common tool in on-chip biodetection systems  and research in this field is improving fast. Mostly whole cells are analyzed by magnetophoresis, because binding of magnetic particles to cells change their magnetophoretic behavior . We developed a technology which is able to detect the load of a magnetic particle by forcing the particle to describe certain trajectories with switching magnets. This allows us to net-focus magnetic beads in a hydrodynamic system. This net-focussing gives us a quantitative insight about how many molecules are bound to the particle surface. The overall system is small enough to use it as a point of care device to detect proteins and nucleic acids. The usage of magnetic beads for our detection additonally combines two process steps, purification and analysis. Therefore, only few process steps are required to obtain a result out of a unpurified sample in which a target molecule should be specified.
Generation neural stem cells from neurosphere –drived bone marrow steromal cells using bioactive substance TNT?potential use for spinal cord injury
Shefa neuroscience research center, Khatam Al-Anbia Hospital
Introduction ?one of the recent promising therapy in spinalcord injury is the use of cytotherapy in order to replace the lost neurons and glial cells.Neural stem cells can be differentiate into all cell types of spinal cord.neural stem cells (NSCs) are cells that maintain the capacity to differentiate into brain-specific cell types, and may also replace or repair diseased brain tissue. NSCs can be directly isolated from adult nervous tissue or derived from embryonic stem cells that these cells are many ethical and limited with their use. Therefore, adult stem cells from the bone marrow stroma have been proposed as an alternative source. Here, we describe the efficient conversion of bone marrow stromal cells (BMSC) into a neural stem cell-like under the influence of a factor inducing non-toxic bioactive substance TNT .
Material & Method
BMSCs were isolated from the femurs and tibias of rat and then culture these cells .the cells induced with bioactive substance TNT. That these cells grow in neurosphere-like structures and then to neural stem cells. these cells evaluated by using immunocytochemistery and RT-PCR technic.
The neurosphere-like structures, express high levels of early neuroectodermal markers, such as the genes SOX2,OCT4,NANOG,NeuroD and nestin. Also ,NSCs express the genes NF68 ,NF200, nestin and by using RT-PCR express the genes SOX2,OCT4,NANOG,NeuroD bioactive substance TNT factor can be a good alternative as a non-toxic substance is to induce neuron. NSCs may therefore ultimately help to treat neurodegenerative diseases.
BMSCs trans-differentiated into NSCs which be used to trent spinal cord injury.
Production of neurosphere with high passages (57 passages) from bone marrow stem cells (BMSC) using Bioactive substance TNT?a promising protocol for multiple treatment modality
Shefa neuroscience research center, Khatam Al-Anbia Hospital
Introduction:Cell therapy is an option in replacing the lost cells in the injured spinal cord. Source of patients need multiple delivery of cells in order to achieve the wanted results.
Stem cells play an essential role during the processes of embryonic development and throughout adulthood. bone marrow stem cells can be give rise to muscle, hepatic and brain cells such as glial cells and neural cells. bone marrow stromal cells are one of the kinds of stem cells, that can be differentiation in to neurosphere. More previously study reported are complicated methods and involve the used toxic factors , Here, we reported a non toxic and efficiented method of rat MSCs into neurosphere that neurosphere can be differentiated in to glial and neural cells.Materials & method:Primary rat BMSCs were isolated from tibias and Femurs of Wistar rats (200–300 g), BMSCs isolation and MSCs cultured by DMEM/F12 medium supplemented with 10% fetal bovine serum. These cells evaluated by specific markers of MSCs and BMSCs such as : bioactive substance TNT and then MSCs differentionated in to neurosphere in 4 group that these groups compared fram morphology, this diffrentionation do by non toxic factor :1:1M 2: 0.1M ,3:0.01 M ,4:0.25M . by dose response and time course at 72 hours. Diameter and numbere of This neurosphere evaluated every day in 4 group.Result:MSCs isolation from BMSCs then evaluated by immunocytochemistry that expressed CD90 ( 80%)CD44(70%)fibronectin and negative marker CD45. Diameter and number of Neurosphere by 0.1 ng/ml was optimal dose for expansion. Then these cells evaluated by neuroectodermal markers such as nestin and NF 68 That expressed >80 % and this data approved by RT-PCR. this study developeded a simplified, efficient, and nontoxic approach by lowest factors which derives a large number of neurospheres from bone marrow stromacells. With our newly devised approach.Counclution:Cell therapy is an option in replacing the lost cells in the injured spinal cord. Source of patients need multiple delivery of cells in order to achieve the wanted results.
Molecular Predictors of the Outcome for Adjuvant Chemotherapy in Egyptian High Risk Female Breast Cancer Patients
Faculty of Medicine, Alexandria University
The aim of the present work was to study the predictive value of estrogen receptors (ER), progesterone receptors (PR) proteins, topoisomerase two alpha (TOPOIIa) gene aberrations (amplification or deletion) and breast cancer 1, early onset (BRCA1)gene methylation for the outcome of 5-fluorouracil / Adriamycin / cyclophosphamide (FAC) adjuvant chemotherapy in Egyptian high risk female breast cancer patients. The present retrospective cohort study included fifty high risk female breast cancer patients (according to St Gallen guidelines 2007) with operable breast cancer. Log rank survival analysis showed that TOPOIIa gene aberrations, methylated BRCA1 gene, negative ER protein and negative ER/PR proteins states were associated with significantly superior disease free survival (DFS) rates after FAC therapy. Cox regression analysis showed that ER protein and BRCA1 gene methylation states might be independent predictors for the outcome of FAC adjuvant chemotherapy while TOPOIIa gene state mightn't. However, if ER protein and BRCA1 gene methylation states can be used in tailoring chemotherapy or not, further studies have to be done on a bigger number of cases with longer follow-up period. Additionally, large-scale prospective studies will be needed to clearly define TOPOIIa gene and PR protein predictive values.
An Emerging Revolution in Automated 3D Cell Culture
Merck Research Laboratories,University of Virginia, BioCell Cubed
Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.
Development of a Lab-on-a-Chip for the Characterization of Human Cells
ARC and Nano-Systemtechnologies
Cell chips are developed to continuously monitor mammalian cell population dynamics in a non-invasive manner. In the presented work we describe the design, fabrication and characterization of a lab-on-a-chip for quantitative cell analysis.
Bio-analytic silicon chips for the detection of developmentalneurotoxic effects of chemicals and drugs in the context of the European REACH program
University of Rostock, Bionas GmbH
Bio-analytic silicon chips can be used to investigate stem cell differentiation into neurons and for the in vitro on-line monitoring of cellular reactions under well controlled experimental conditions. Chip systems allow for a parallel, label-free and non-invasive measurement of different parameters by CMOS silicon microsensors by the application of potential neurotoxic and developmental neurotoxic substances.
RNA Interference in Mammalian Cells Using low siRNA Concentrations
We have developed a transfection reagent, HiPerFect Transfection Reagent, which allows efficient gene knockdown with siRNA concentrations from 1 nM–10 nM, depending on the cell type and siRNA used. HiPerFect Transfection Reagent has been tested and validated for many cell types, including primary cells. Effective knockdown in primary cells demonstrates that HiPerFect Transfection Reagent ensures low cytotoxicity levels.
SLAS2014 will feature all the great scientific education, programming, intelligent network building and new products and services you have come to expect from SLAS. Make plans now to join us!
CellTech 2014 will feature an integrated program of tracks designed to describe the latest trends in analysis of cells, cell-based assays and high-content phenotypic assays. This 3 track conference will also feature Flow Cytometry and Bioprocessing, which includes up-stream and down-stream segments.
2014 Ocean Sciences Meeting
Ocean Sciences Meeting
In February 2014, ASLO, TOS, and AGU will convene the biennial Ocean Sciences Meeting in Honolulu, Hawaii. Scientists from around the world will converge on this beautiful city to exchange knowledge and discuss issues covering the broad spectrum of marine science disciplines, including physical, biological, chemical and geological oceanography, technology and multidisciplinary topics.
16th Ottawa Conference & 12th Canadian Conference on Medical Education (CCME) April 25-29,2014
As one integrated conference, we are bringing Canada’s preeminent medical education conference together with the leading international conference on the assessment of clinical competence.