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Jędrzej Szymański wins Best Poster Award at Bioimaging: From Cells to Molecules 2016
Jędrzej Szymański wins Best Poster Award at Bioimaging: From Cells to Molecules 2016

With the use of Fluorescence Correlation Spectroscopy (FCS) and cellular viscosity model we can quantify the diffusion coefficient of Drp1 in the cytoplasm and the extent of its aggregation. Using different mutants of Drp1 and a stable cell line expressing GFP-Drp1 it can be shown that Drp1 forms tetramers or higher oligomers in the cytoplasm.
Poster
Insight Into The Mitochondrial Fission from The Diffusion Of Drp1 Protein In The Living Cell.
Insight Into The Mitochondrial Fission from The Diffusion Of Drp1 Protein In The Living Cell.
Bernadeta Michalska, Jerzy Duszyński, Jędrzej Szymański
Nencki Institute of Experimental Biology PAS
Poster Views: 2,709
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Poster Abstract
Protein Drp1 is a GTPase involved in the process of mitochondrial fission. Upon fission signals in the cell, Drp1 intermediate assemblies form oligomeric ring-like structures which wrap around mitochondria and then constrict thanks to the energy gained from GTP hydrolysis. The assembly of the fission complex may progress through different Drp1 oligomeric states. With the use of Fluorescence Correlation Spectroscopy (FCS) and cellular viscosity model we can quantify the diffusion coefficient of Drp1 in the cytoplasm and the extent of its aggregation. Using different mutants of Drp1 and a stable cell line expressing GFP-Drp1 it can be shown that Drp1 forms tetramers or higher oligomers in the cytoplasm. Observations made using spinning disc confocal microscopy showed that Drp1 forms stable large oligomers which can move along mitochondria and the formation of the Drp1 ring on the mitochondrion does not necessarily lead to fission event.

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