Award Winner
« Back
Lisa Mutschelknaus wins Best Poster Award at Extracellular Vesicles 2016
Lisa Mutschelknaus wins Best Poster Award at Extracellular Vesicles 2016

This study indicates a functional role for exosomes in the response of tumor cells to therapeutic radiation exposure, suggests that radiotherapy modified exosomes influence the cancer progression and encourages that exosomes might be a useful tool to improve therapy strategies.
Poster
Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation
Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation
Lisa Mutschelknaus, Carsten Peters, Klaudia Winkler, Ramesh Yentrapalli, Theresa Heider, Michael J. Atkinson, Simone Moertl
Helmholtz Zentrum München, Institute of Radiation Biology, Neuherberg, Germany / Department of Chemistry, Technical University of Munich, Munich, Germany
Poster Views: 4,015
view poster »
Poster Abstract
Exosomes were isolated from conditioned medium of irradiated as well as non-irradiated head and neck cancer cells (BHY and FaDu) by serial ultracentrifugation. Quantification by NanoSight technology indicates an increased exosome release from irradiated compared to non-irradiated cells 24 hours after treatment. To test whether the released exosomes influence the radiation response, exosomes isolated from non-irradiated and irradiated donor cells were transferred on non-irradiated and irradiated recipient cells. We find an enhanced uptake of exosomes, when transferred to irradiated recipient cells compared to the transfer to non-irradiated cells. Functional analyses after exosome transfer indicate that exosomes increase not only the proliferation but also the survival after irradiation in recipient cells. These findings mesh with an increased DNA double strand break repair after the transfer of exosomes isolated from irradiated cells.
Our results demonstrate that radiation increases exosome abundance and influences their effects on recipient cells. This study indicates a functional role for exosomes in the response of tumor cells to therapeutic radiation exposure, suggests that radiotherapy modified exosomes influence the cancer progression and encourages that exosomes might be a useful tool to improve therapy strategies.


Related Posters


MODEL-AD: Bioinformatics and Data ManagementMODEL-AD: Bioinformatics and Data Management
Bruce Lamb1, Michael Sasner2, Andrew Saykin1, Lara Mangravite3, Gregory Carter2

MODEL-AD: The Disease Modeling Project
M.Sasner1, A. Oblak2, H. Williams1, G. Howell1, B.T. Lamb2 and the MODEL-AD consortium

MODEL-AD: Genetic models of late-onset Alzheimer’s disease
*A. OBLAK1,2, G. CARTER3, G. R. HOWELL3, B. LOGSDON4, L. MANGRAVITE4, K. NHO2, L. OMBERG4, K. D. ONOS3, V. PHILIP3, C. PREUSS3, S. J. SUKOFF RIZZO3, M. SASNER3, L. SHEN1,2, A. J. SAYKIN1,2, P. TERRITO1,2, A. UYAR3, H. WILLIAMS3, B. T. LAMB1,5

ESTUDIO ANALÍTICO DE LA CALIDAD Y VALORACION NUTRICIONAL DE LALECHE HUMANA DE LAS MADRES DONANTES EN LA ZONA NORTE DEL TOLIMA
Kevin Fernando Romero Acosta; Monica Patricia Osorio Tangarife; Guillermo Salamanca Grosso

Preclinical drug screening in new generation Alzheimer’s disease mouse models: The MODEL-AD Consortium Strategy
P.R. Territo1, S.J. Sukoff Rizzo2, K. Onos2, J.A. Meyer1, J. Peters1, S.C. Persohn1, B.R. McCarthy1, A.A. Riley1, S. Quinney1, D. Jones1, M. Sasner2, G. Howell2, H. Williams2, A.J. Oblak1, B.L. Lamb1 and the MODEL-AD consortium