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YoungWon Koo wins Best Poster Award at International Bioprinting Congress 2015
YoungWon Koo wins Best Poster Award at International Bioprinting Congress 2015

In this study, PZT assists the 3D printing machine in order to reduce the shear stress in a nozzle via micro-scale vibration.
Poster
Enhanced Cell Viability in Bio-printing Process via Piezoelectric Transducer (PZT)-assisted 3D Printing Method
Enhanced Cell Viability in Bio-printing Process via Piezoelectric Transducer (PZT)-assisted 3D Printing Method
YoungWon Koo1, YongBok Kim1, Hyeongjin Lee1, SeungHyun Ahn1, Frederick Mun2, Seokho Kevin Cho3, GeunHyung Kim1
1Department of Bio-Mechatronic Eng., Sungkyunkwan University, Suwon, South Korea 2Department of Biological Science, Carnegie Mellon University, Pittsburgh, USA 3Okemos High School, Okemos, USA
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Poster Abstract
3D printing has been a widely used technology in various areas, which include manufacturing, art, engineering, and medicine, taking center stage under the international spotlight. Tissue engineering became one of the great applications of 3D printing for the fabrication of 3D medical tissues through bio-printing. 3D bio-printing can be divided into 3 big methods; inkjet, micro-extrusion, and laser-assisted bio-printing. Micro-extrusion method is an inexpensive and comparatively easy way to fabricate 3D structures, having drawbacks such as low cell viability and low printing speed on the other hand. In this work, advanced cell-laden structures have been constructed with higher cell viability when piezoelectric transducer (PZT) was applied to the micro-extrusion method to overcome its conventional demerits. Usual usage of PZT in bio-printing is to vibrate bio-ink in micro-scale for dropping the ink in inkjet method. In micro-extrusion method, however, the assisted PZT can enhance the printing efficiency by decreasing the shear stress in nozzles due to the PZT effect that lower the viscosity of the bio-ink via its micro-scale vibration. In this study, 3, 5, and 7 weight % of alginate cross-linked with 0.5 weight % of CaCl2 was printed with pressures 190, 340, and 440 kPa, respectively. The effectiveness of PZT was examined through the PZT frequency range 0 to 500Hz (0, 100, 200, and 500Hz). The in vitro biocompatibilities of the cell-laden structure were examined using osteoblast-like cells (MG63). Cell viability (live/dead) and MTT assay were assessed. As a result, PZT has effectiveness of the printability and cell viability in cell printing and this can be supplementation for low cell viability in high pressures, which is a demerit of the micro-extrusion method.

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