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143:Canine Chronic Ulcerative Stomatitis: Characterization of the Oral Microbiome[AAOM2021]
EP36046
Poster Title: 143:Canine Chronic Ulcerative Stomatitis: Characterization of the Oral Microbiome[AAOM2021]
Submitted on 29 Mar 2021
Author(s): Anderson JG*, Paster BJ^, Kokaras A^, Chen T^
Affiliations: Sacramento Veterinary Dental Services, United States of America
This poster was presented at 2021 American Academy of Oral Medicine Virtual Conference
Poster Views: 223
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Poster Information
Abstract: Hypothesis: Various studies have described the oral microbiome of dogs in health and early periodontitis yielding distinct differences from the human oral microbiome. As well, there has been a significant transition in understanding away from the “specific plaque hypothesis” to the keystone plaque hypothesis which holds that certain low-abundance microbial pathogens can orchestrate inflammatory disease by remodelling a normally benign microbiota into a dysbiotic one in a susceptible host. We hypothesized that Canine Chronic Ulcerative Stomatitis (CCUS) lesions are characterized by an altered microbiome, which likely includes potentially pathogenic bacteria.

Objectives:
1. Determine the oral microbiome of the CCUS mucosal lesion
2. Compare the oral microbiome in CCUS lesional mucosal tissue, to plaque of opposing teeth, and to a normal mucosal site in CCUS, and to healthy mucosal controls

Plaque Samples: Sterile paper point plaque samples were obtained from 59 CCUS mucosal lesions and abutting tooth surface, normal CCUS site, and ten healthy mucosal controls.
Procedures: DNA isolation, next generation sequencing, bioinformatics/biostatistics were performed at The Forsyth Institute.
Results: Using a program called LefSe, a two-tiered statistical analysis, there was a statistically significant difference comparing mucosal samples of CCUS lesions to normal sites. Those species prevalent on the mucosal ulcers were putative periodontal pathogens. Further, there was a good distribution of specific periodontopathogens that had similar patterns on mucosal ulcer and opposing tooth surface.

Conclusions: CCUS lesions are characterized by an altered microbiome, with decreased diversity, and increased abundance of potentially pathogenic bacteria. Dysbiosis is suspected.
Summary: This study details the microbiome in CCUS and provides further insights into its pathogenesis and proposed treatment of dogs with this painful
condition. The knowledge gained enhances our ability to medically treat this condition, rather than extracting functional teeth.

Ask the author questions about this poster:jgadvm@gmail.com
References: Anderson JG, Peralta S, Kol A, Kass PH, Murphy B. Clinical and Histopathologic Characterization of Canine Chronic Ulcerative Stomatitis. Vet Pathol. 2017;54(3):511-9.

Anderson JG, Kol A, Bizikova P, Stapelton BP, Ford K, Villarreal A, et al. Immunopathogenesis of canine chronic ulcerative stomatitis. PLoS One. 2020;15(1):e0227386.

Chen T, Yu WH, Izard J, Baranova OV, Lakshmanan A, Dewhirst FE. The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database (Oxford). 2010;2010:baq013.

Dewhirst FE, Klein EA, Thompson EC, Blanton JM, Chen T, Milella L, et al. The canine oral microbiome. PLoS One. 2012;7(4):e36067.

Hajishengallis G, Darveau RP, Curtis MA. The keystone pathogen hypothesis. Nat Rev Microbiol. 2012;10(10):717-25.
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