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163:Association Between The Expression Of Apoptotic (Bcl-2/Bax), Cell Proliferation (Ki-67), Angiogenic (iNOS) Oncoproteins And Oxidative Stress Markers In Tissue Samples Of Patients With Oral Leukoplakia And Oral Squamous Cell Carcinoma[AAOM2021]
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Poster Title: 163:Association Between The Expression Of Apoptotic (Bcl-2/Bax), Cell Proliferation (Ki-67), Angiogenic (iNOS) Oncoproteins And Oxidative Stress Markers In Tissue Samples Of Patients With Oral Leukoplakia And Oral Squamous Cell Carcinoma[AAOM2021]
Submitted on 29 Mar 2021
Author(s): Kumar Chandan Srivastava, Deepti Shrivastava
Affiliations: College of Dentistry, Jouf University, Saudi Arabia
This poster was presented at 2021 American Academy of Oral Medicine Virtual Conference
Poster Views: 211
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Poster Information
Abstract: Objectives: To evaluate and correlate the expressions of Bcl-2/Bax, Ki-67, iNOS (oncoproteins) with oxidative stress markers (TBARS, GSH, and GPx) in patients with various clinicopathological stages of oral leukoplakia (OL) & oral squamous cell carcinoma (OSCC).

Methods: A case-control study with three study groups were designed. Group A and B (case groups) comprised thirty, each newly diagnosed patient of OL and OSCC. Group C (control) included thirty, age and gender matched healthy subjects. After clinical examination, we collected tissue samples from both case and control groups. We determined clinical staging and histopathological grading for every patient in the case groups. We performed a spectrophotometric analysis to assess the tissue levels of thiobarbituric acid reactive substances (TBARS), glutathione (GSH), and glutathione peroxidase (GPx). Later, immunohistochemical analysis was conducted to assess the expression of various oncoproteins including Ki-67, Bcl-2/Bax, and i-NOS (inducible nitric oxide synthase). Depending on the data, the Kruskal–Wallis /One-Way ANOVA test was used to make statistical comparisons between the study groups. Pearson / spearmen test was employed to assess correlation among the oxidative markers and oncoproteins.

Results: A Significant reduction (P<.001) in TBARS, along with the elevated levels (P<.001) of GSH and GPx enzymes, was reported in both case groups when compared with control. These oxidative markers displayed a similar trend (P<.05) with the advancing clinical stages and grades of OL and OSCC.

Compared with the control group, over-expression (P<.05) of Bcl-2, Ki-67, and iNOS was observed in OL and OSCC. Conversely, reduced expression of Bax (P<.05) was reported in the case groups compared to the control group subjects.

A positive correlation (P<.001) was reported between oncoproteins (Bcl-2, Ki-67, and i-NOS) and antioxidant enzymes, while a negative correlation (P<.001) was observed with TBARS.

Conclusions: Reduced lipid peroxidation and increased activity of antioxidant enzymes possibly provide a suitable environment for tumor growth. An increase in expression of Bcl-2, iNOS, and Ki-67 with the advancing stages and grades of OL and OSCC, support the progression of the tumor. Although there is evidence of an association between oxidative markers and oncoproteins, longitudinal studies are required to establish a causal relationship.
Summary: Objectives: To evaluate and correlate the expressions of Bcl-2/Bax, Ki-67, iNOS (oncoproteins) with oxidative stress markers (TBARS, GSH, and GPx) in patients with various clinicopathological stages of oral leukoplakia (OL) & oral squamous cell carcinoma (OSCC).

Ask the author questions about this poster:drkcs.omr@gmail.com
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