We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP30775
Poster Title: 2D FT0ICR MS/MS analysis of IgG1
Submitted on 10 Oct 2019
Author(s): Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1
Affiliations: 1. ICR lab, University of Warwick, CV4 7AL, Coventry, UK 2. 3IGBMC, Illkirch Graffenstaden , France 3. UCB, SL1 3WE, Slough, UK
Poster Views: 102
Please be aware that this Poster is viewable on an external site.
Submitted on 10 Oct 2019
Author(s): Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1
Affiliations: 1. ICR lab, University of Warwick, CV4 7AL, Coventry, UK 2. 3IGBMC, Illkirch Graffenstaden , France 3. UCB, SL1 3WE, Slough, UK
Poster Views: 102
Please be aware that this Poster is viewable on an external site.
Abstract: Recombinant monoclonal antibodies and derivatives are widely used as therapeutic drugs They are susceptible to post translational modifications that could occur during the manufacturing process and storage, resulting in product related impurities PTMs can change the efficacy, toxicity, or the clearance of the antibody therefore they need to be well monitored.Summary: Recombinant monoclonal antibodies and derivatives are widely used as therapeutic drugs They are susceptible to post translational modifications that could occur during the manufacturing process and storage, resulting in product related impurities PTMs can change the efficacy, toxicity, or the clearance of the antibody therefore they need to be well monitored.References: [1] Amster , I. J., Fourier transform mass spectrometry. J. Mass Spectrom. 1996, 31 (12), 1325 1337
[2] Tsybin , Y. O.; Witt, M.; Baykut , G.; Kjeldsen , F.; Håkansson , P., Combined infrared multiphoton dissociation and electron capture dissociation with a hollow electron beam in Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun . Mass Spectrom. 2003, 17 (15), 1759 1768.
[3] Pfändler , P.; Bodenhausen , G.; Rapin , J.; Houriet , R.; Gäumann , T., Two dimensional Fourier transform ion cyclotron resonance mass spectrometry. Chem. Phys. Lett. 1987, 138 (2 3), 195 200
[4] Flora, J. W.; Muddiman , Selective , sensitive, and rapid phosphopeptide identification in enzymatic digests using ESI FTICR MS with infrared multiphoton dissociation. Anal Chem. 2001 , 73 (14), 3305 3311
[5] Flora, J. W.; Muddiman , Gas phase ion unimolecular dissociation for rapid phosphopeptide mapping by IRMPD in a penning ion trap: An energetically favored process. JACS 2002
[2] Tsybin , Y. O.; Witt, M.; Baykut , G.; Kjeldsen , F.; Håkansson , P., Combined infrared multiphoton dissociation and electron capture dissociation with a hollow electron beam in Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun . Mass Spectrom. 2003, 17 (15), 1759 1768.
[3] Pfändler , P.; Bodenhausen , G.; Rapin , J.; Houriet , R.; Gäumann , T., Two dimensional Fourier transform ion cyclotron resonance mass spectrometry. Chem. Phys. Lett. 1987, 138 (2 3), 195 200
[4] Flora, J. W.; Muddiman , Selective , sensitive, and rapid phosphopeptide identification in enzymatic digests using ESI FTICR MS with infrared multiphoton dissociation. Anal Chem. 2001 , 73 (14), 3305 3311
[5] Flora, J. W.; Muddiman , Gas phase ion unimolecular dissociation for rapid phosphopeptide mapping by IRMPD in a penning ion trap: An energetically favored process. JACS 2002
Ask the author a question about this poster.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Streamlining Biologics Development with the Expi Expression Systems
Chao Yan Liu, Jian Liu, Wanhua Yan, Kyle Williston, Kenneth Thompson, Maya Yovcheva, Sara Barnes, Mark Bundy, Melissa Cross, Katy Irvin, Joaquín Canay, Mary Reynolds, Natasha Lucki, Henry Chiou, Andrew Campbell, Jonathan Zmuda
ExpiSf™ Expression system: A Chemically Defined Baculovirus-Based System for Enhanced Protein and Virus Production in Sf9 Cells
Kenneth Thompson, Maya Yovcheva, Sara Barnes, Melissa Cross, Katy Irvin, Natasha Lucki, Henry Chiou, and Jonathan Zmuda
Optimized Expression of Membrane Proteins in the Expi293 and ExpiCHO Expression Systems: New Tools for Difficult to Express Proteins
Chao Yan Liu1, Jian Liu1, Wanhua Yan1, Sam Stepnowski1 ,Kyle Williston1, Katy Irvin1, Chetana Revankar2, Natasha Lucki2, Henry Chiou2, Jonathan Zmuda1. Thermo Fisher Scientific, 1Frederick, MD, U.S.A, 2Carlsbad, CA, U.S.A
High speed untargeted 4D-lipidomics LC-MS/MS workflows with Parallel Accumulation Serial Fragmentation (PASEF)
Ulrike Schweiger-Hufnagel1, Aiko Barsch1, Sven W. Meyer1
TMT Quantification for Shotgun Proteomics on a Trapped-Ion-Mobility quadrupole-Time-Of- Flight mass spectrometer (TIMS-QTOF) powered by Parallel-Accumulation and Serial- Fragmentation (PASEF)
Matt Willetts1, Shourjo Ghose1 Heiner Koch2, Scarlet Koch2, Markus Lubeck2, Gary Kruppa1