« Back
MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Poster Title: MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Submitted on 15 Feb 2019
Author(s): Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1
Affiliations: 1.Bruker Japan, K.K., Yokohama, Japan 2.Doshisha University, Faculty of Life and Medical Systems, Kyoto, Japan 3.Georges-Pombidou European Hospital, Anatomy-Pathology, Paris, France 4.National Cerebral and Cardiovascular Research Center, Osaka, Japan
Poster Views: 691
View poster »

Please be aware that this Poster is viewable on an external site.

Poster Information
Abstract: Tissue Preparation: FFPE tissue samples were transferred fromGeorges-Pombidou European Hospital, (GPEH) Paris, France. Tissue sections were prepared from FFPE tissue samples according to modified procedures as follows: 1) Dewaxing 2) On-tissue digestion using trypsin as a protease 3) Deposition of MALDI matrix with Image Prep and HTXTM-sprayer with or without manual sprayer for better ionization.

Immunohistochemistry: Anti TTR antibody and anti AL antibody (Santa Cruze Biotechnology, Inc.) were used for IHC. Amyloid staining were performed with Sirius red.

Mass Spectrometry: Matrix coating: DHB in 50% MeOH, Image Prep and or manually sprayed with airbrush; Standard trypsin deposition protocol; MALDI: Rapiflex; flex Imaging 5.0 software. (Bruker Daltonics). Tissues were stained by H&E. PCA and pLSA analysis of mass spectra was performed with SCiLSLab 2016B software. (Bruker Daltonics).
Summary: Here we have applied a comprehensive Matrix-assisted laser desorption/ionization (MALDI) Mass spectrometry (IMS) approach to formalin-fixed paraffin-embedded(FFPE)tissue samples that have been acquired from the EMB conducted after the transplantations at Hôpital Européen Georges-Pompidou in Paris, France.References: 1) Circulation. Lupy A. Et al., 2017
2) Acta Neuropatho Comm. Kakuda et al., 2017
3) Clinical Biochemistry. Nakanishi et al., 2013
4) Circulation Reserach Nour-Eldine W., 2018
Report abuse »
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters

HIV 30 Years of Themes
Eman Momtaz under the supervision of Dr reem Abu Assi

The Optimum Food for AIDS Patients’ Health Support
Mhamd salih imad hamdi under the supervision of Dr reem Abu Assi

FXa Anticoagulant Bioactive Proteins Derived from Scorpion Venom
Meng Li,1 Yuko P. Y. Lam,1 Peng Chen,2 Remy Gavard,1 Cookson K. C. Chiu,1 Christopher A. Wootton,1 Tomos E. Morgan,1 Qiong Wu,2 Mark P. Barrow,1 Hongzheng Fu,2 Peter B. O’Connor1

The Inhibition Pathways of Human Islet Amyloid Polypeptide
Yuko P. Y. Lam1; Cookson K.C. Chiu1; Christopher A. Wootton1; Ji-Inn Song1; Meng Li1; Ian Hands-Portman2; Mark P. Barrow1; and Peter B. O'Connor1

Etiological diagnostics of cardiomyopathies in the cardiology unit of the HUEH internal medicine department during the period from 8 January 2014 to 14 June 2014
*Axler JEAN PAUL; **Lesly Ed. ARCHER; *Raema Mimrod JEAN; *Gerald VERNELUS; *Rodolph MALEBRANCHE