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MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Poster Title: MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Submitted on 15 Feb 2019
Author(s): Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1
Affiliations: 1.Bruker Japan, K.K., Yokohama, Japan 2.Doshisha University, Faculty of Life and Medical Systems, Kyoto, Japan 3.Georges-Pombidou European Hospital, Anatomy-Pathology, Paris, France 4.National Cerebral and Cardiovascular Research Center, Osaka, Japan
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Abstract: Tissue Preparation: FFPE tissue samples were transferred fromGeorges-Pombidou European Hospital, (GPEH) Paris, France. Tissue sections were prepared from FFPE tissue samples according to modified procedures as follows: 1) Dewaxing 2) On-tissue digestion using trypsin as a protease 3) Deposition of MALDI matrix with Image Prep and HTXTM-sprayer with or without manual sprayer for better ionization.

Immunohistochemistry: Anti TTR antibody and anti AL antibody (Santa Cruze Biotechnology, Inc.) were used for IHC. Amyloid staining were performed with Sirius red.

Mass Spectrometry: Matrix coating: DHB in 50% MeOH, Image Prep and or manually sprayed with airbrush; Standard trypsin deposition protocol; MALDI: Rapiflex; flex Imaging 5.0 software. (Bruker Daltonics). Tissues were stained by H&E. PCA and pLSA analysis of mass spectra was performed with SCiLSLab 2016B software. (Bruker Daltonics).
Summary: Here we have applied a comprehensive Matrix-assisted laser desorption/ionization (MALDI) Mass spectrometry (IMS) approach to formalin-fixed paraffin-embedded(FFPE)tissue samples that have been acquired from the EMB conducted after the transplantations at Hôpital Européen Georges-Pompidou in Paris, France.References: 1) Circulation. Lupy A. Et al., 2017
2) Acta Neuropatho Comm. Kakuda et al., 2017
3) Clinical Biochemistry. Nakanishi et al., 2013
4) Circulation Reserach Nour-Eldine W., 2018
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