We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP31456
Poster Title: Novel MALDI-TOF MS Workflow for Ultrahigh-Throughput Screening of Different Analytes at Each Position on a Plate
Submitted on 31 Mar 2020
Author(s): Sergei Dikler
Affiliations: Bruker Scientific, LLC, Billerica, MA
Poster Views: 375
Please be aware that this Poster is viewable on an external site.
Submitted on 31 Mar 2020
Author(s): Sergei Dikler
Affiliations: Bruker Scientific, LLC, Billerica, MA
Poster Views: 375
Please be aware that this Poster is viewable on an external site.
Abstract: MALDI-TOF mass spectrometry was established as a label-free ultrahigh-throughput screening approach for biochemical assays where substrates and products were small molecules, peptides and proteins. In this case the same list of analytes was monitored at every position on a MALDI plate. More recently, we extended this approach to screening of synthetic chemical reactions, which required monitoring different small molecule starting materials and products at every position on a MALDI plate (Figure 1). This necessitated the development of a novel software workflow and optimization of data processing methods for a dramatically increased number of analytes. This work focuses on application of the new Synthesis Screening workflow not only to screening of chemical reactions but also to screening of a custom library of small molecule pharmaceuticals.Summary: The Synthesis Screening module in the MALDI PharmaPulse software was used to screen a custom library of 41 small molecule pharmaceuticals.
The automated runs using a MALDI plate in 384 format had less than one failed plate position Results per run on average.
The automated runs using a MALDI plate in 384 format had less than one failed plate position Results per run on average.
Ask the author a question about this poster.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Health vision 2030: An Era of Evolution and Revolution in India
GAURAV JAIN
Combining Imaging and OMICs: MALDI guided SpatialOMx probes proteomic mass profiles of breast cancer tumor subpopulations
Janina Oetjen 1 , Romano Hebeler 1 , Frédéric Dewez 2 , Corinna Henkel 1 , Benjamin Balluff 2 , Ron Heeren 2
FXa Anticoagulant Bioactive Proteins Derived from Scorpion Venom
Meng Li,1 Yuko P. Y. Lam,1 Peng Chen,2 Remy Gavard,1 Cookson K. C. Chiu,1 Christopher A. Wootton,1 Tomos E. Morgan,1 Qiong Wu,2 Mark P. Barrow,1 Hongzheng Fu,2 Peter B. O’Connor1
The Inhibition Pathways of Human Islet Amyloid Polypeptide
Yuko P. Y. Lam1; Cookson K.C. Chiu1; Christopher A. Wootton1; Ji-Inn Song1; Meng Li1; Ian Hands-Portman2; Mark P. Barrow1; and Peter B. O'Connor1
2D FT-ICR MS/MS analysis of IgG1
Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1