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Combining Imaging and OMICs: MALDI guided SpatialOMx probes proteomic mass profiles of breast cancer tumor subpopulations
Poster Title: Combining Imaging and OMICs: MALDI guided SpatialOMx probes proteomic mass profiles of breast cancer tumor subpopulations
Submitted on 03 Apr 2020
Author(s): Janina Oetjen 1 , Romano Hebeler 1 , Frédéric Dewez 2 , Corinna Henkel 1 , Benjamin Balluff 2 , Ron Heeren 2
Affiliations: 1 Bruker Daltonik GmbH, Bremen, Germany; 2 Maastricht MultiModal Molecular Imaging Institute (M4I), University of Maastricht, Maastricht, The Netherlands
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Poster Information
Abstract: SpatialOMx workflow provides detailed in situ characterization of bioactivity across different subtypes of tumor microenvironment.

MALDI Imaging and analysis guides laser microdissection to excise distinctive tissue subtypes.

timsTOF fleX can perform both MALDI Imaging and LC MS/MS.

timsTOF fleX can provide in depth proteomics analysis of minute amounts of dissected tissue using PASEF.
Summary: While MALDI Imaging can provide spatial information on molecular distribution in tissue, the technique lacks capability for in depth characterization on any discoverable biomarkers. SpatialOMx is a novel workflow that unifies MALDI Imaging with traditional OMICs based LC/MS techniques to provide comprehensive biochemical mass profiling of biological tissue.References: [1] Meier F et al 2015 J Proteome Res 14 5378 5387
[2] Longuespée R et al 2016 Methods 104 154 162
[3] Dewez F, et al 2019 Anal Bioanal Chem 411 5647
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