We've updated our Privacy Policy to make it clearer how we use your personal data.
We use cookies to provide you with a better experience, read our Cookie Policy
EP29484
Poster Title: A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells
Submitted on 10 Dec 2018
Author(s): Maya Yovcheva, Sara Barnes, Kenneth Thompson, Melissa Cross, Katy Irvin, Mintu Desai, Natasha Lucki, Henry Chiou, Jonathan Zmuda
Affiliations:
Poster Views: 546
Submitted on 10 Dec 2018
Author(s): Maya Yovcheva, Sara Barnes, Kenneth Thompson, Melissa Cross, Katy Irvin, Mintu Desai, Natasha Lucki, Henry Chiou, Jonathan Zmuda
Affiliations:
Poster Views: 546
Abstract: Here, we present data on the performance of a novel Sf9-based Baculovirus expression system based upon a yeastolate-free, animal origin-free, chemically-defined, high-density culture medium that allows for Sf9 cells to reach densities nearly twice as high as those attained in traditional yeastolatecontaining media. Sf9 cells adapted to grow to high densities in the yeastolate-free media were generated and a new, high-efficiency Bacmid transfection reagent was developed to allow for the generation of high titer baculovirus stocks. Together, with the addition of protein expression enhancer these improvements allow for the optimization of a new expression protocol that takes advantage of the high cell densities achievable with the new chemically-defined medium and adapted Sf9 cells, as well as high multiplicity of infection (MOI), to significantly improve protein titers and enable lot -to- lot consistency of both cell growth and protein expression in a defined media formulation.Summary: Here, we present data on the performance of a novel Sf9-based Baculovirus expression system based upon a yeastolate-free, animal origin-free, chemically-defined, high-density culture medium that allows for Sf9 cells to reach densities nearly twice as high as those attained in traditional yeastolatecontaining media. References:
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Related Posters
Lower-Order Aberrations and Relaxing Color in Young Chinese Adults
Diana Derval
Single-cell studies: Focus on reverse transcription
Zucha Daniel, Androvic Peter, Kubista Mikael, Valihrach Lukas
Oral Soft Tissue Lumps and Bumps
Dr. Amal Hassan Zakarya, Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh
Potential therapeutic effects of induced pluripotent stem cells on induced salivary gland cancer in experimental rats
Yasmine Alaa El-Din1, Dina Sabry2, Amal Hassan Abdelrahman3, and Safa Fathy3
The effect of Wharton's jelly mesenchymal stem cells conditioned medium on Squamous cell carcinoma cell line.
Moataz El-Kholy (1), Mohamed S. Ayoub (2) and Mohamed H. El-Mallahy (2)