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a comparison of dispense performance of manual pipetting versus automated pipetting for assay development
EP31198
Poster Title: a comparison of dispense performance of manual pipetting versus automated pipetting for assay development
Submitted on 10 Jan 2020
Author(s): Anne F. Hammerstein, David J. Onley, Joby Jenkins, Paul G. Wylie, Sarah Payne
Affiliations: SPT Labtech Ltd, Melbourn Science Park, Melbourn, Rosyton, Hertfordshire, SG8 6HB, UK. 
Poster Views: 121
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Poster Information
Abstract: Every new assay requires significant optimisation (e.g. enzyme and substrate concentrations, cell type and number, buffer constituents, pH, incubation timepoint) for robust performance and assured decision making. Hand pipettes are often utilised for this step, as they can handle a wide range of liquid viscosities that would be challenging for automated dispensers. However, with a manual approach the number of variables per assay is limited, due to the sheer difficulty and time it would take to dispense all desired reagent combinations. This leads to long and iterative assay development cycles.

Here we assess a positive displacement dispensing instrument that incorporates the versatility of a hand pipettor, but in an automated manner to reduce assay development times from weeks to just hours or days.The system uses up to ten independent channels to rapidly dispense liquids into high density plates, without any requirement for liquid classification. Each disposable tip has a low minimum dispense volume of 200nL and a low dead volume, which enables comprehensive miniaturised assay optimisation with precious reagents such as proteins or cells.

In this poster, we compare the performance in dispensing different liquids both by hand and by using the dragonfly®discovery automated pipettor. We also highlight the benefits in its utility in running complex design of experiments leading to faster assay development, in addition to obtaining more robust assays.
Summary: Compared to manual pipetting, this automated dispenser offers to the following benefits -
Robust Dispense
Versatility of a Handheld Pitpette
Gentle on Cells
Increased Assay Development Efficiency
Effortless Assay Transition to HTS
References: We would like to thank S. Gokhin, K. Chen, S. Cerruti, A. Hill and D. Auld (Novartis Institutes for Biomedical Research, Cambridge, MA) for permission to use their data in section 6, previously published as a poster (Gokhinet al., SLAS, 2015)Report abuse »
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