Posters
« Back
A Miniaturized and Automated P450-Glo™ Screening System Using the Echo® Liquid Handler
EP20376
Poster Title: A Miniaturized and Automated P450-Glo™ Screening System Using the Echo® Liquid Handler
Submitted on 18 Dec 2013
Author(s): Jing Wang, Joseph Barco, Tracy Worzella, and Kevin Kershner
Affiliations: Labcyte & Promega
Poster Views: 949
View poster »


Poster Information
Abstract: The CYP3A4 enzyme is the most abundant enzyme in the liver and plays a major role in metabolizing many xenobiotics in humans. The Promega P450-Glo™ CYP3A4 Assay (Luciferin-IPA) offers an extremely sensitive, high throughput and specific luminescence assay for the examination of CYP3A4 inhibition with pooled Human Liver Microsomes. The Echo 555 liquid handler provides precise and accurate acoustic transfer free of cross-contamination risk. Here, we demonstrate optimization of the P450-Glo™ Assay with Human Liver Microsomes (HLM) in low volume format using the Echo 555 liquid handler. We show that the Echo 555 liquid handler can transfer microsomes, inhibitors and substrate in nanoliter volumes, providing a sensitive and robust small scale assay. IC50 results for four known CYP3A4 inhibitors using our miniaturized assay compared favorably to previously reported values in the literature.Summary: Early assessment of cytochrome P450 (CYP) inhibition has become an essential component of drug discovery screening.References: Report abuse »
Creative Commons

Related Posters


A Novel Enzymatic Assay for Determination of Phosphatidylinositol in Biological Samples
Paul Templeton, Kyle C. Schmitt, Grigoriy Tchaga,and Gordon Yan

A Chemically-Defined Baculovirus-Based Expression System for Enhanced Protein Production in Sf9 Cells
Maya Yovcheva, Sara Barnes, Kenneth Thompson, Melissa Cross, Katy Irvin, Mintu Desai, Natasha Lucki, Henry Chiou, Jonathan Zmuda

Development of an in vitro Model System for Newcastle Disease Virus Persistence in Bladder Cancer Cells
Ahmad U1,, Chan SC2, Chau DM1, Chia SL5, Abdullah S1,3, Yusoff K5 & Veerakumarasivam A1,4*

NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL AND CHEMICAL MARKERS
Salamanca Grosso, G.; Osorio Tangafarife, M.P.

The EurOPDX EDIReX project: towards a European Research Infrastructure on patient-derived cancer models
E. Vinolo 1, J.P. Morris 1, D.G. Alférez 2, J. Arribas 3,4,5, C. Bernadó 3,4,5, A. Bertotti 6, A. Bruna 7, A.T. Byrne 8, C. Caldas 7, R.B. Clarke 2, N. Conte 9, R. Corsi 10, S. Corso 6, M. Crespo 3, A. Dahmani 11, V. Dangles-Marie 11, D. Decaudin 11, Z. Dudová 12, A. Fiori 6, S. Giordano 6, M. Hauptsmann 13, M. Hidalgo 14, C. Isella 6, S. de Jong 15, J. Jonkers 13, A. Křenek 12, O. Krijgsman 13, D. Kouřil 12, J.C. Lacal 14, L. Lanfrancone 16, E. Leucci 17, G.M. Mælandsmo 18, E. Marangoni 11, J. Mason 9, M.Th. Mayrhofer 19, A. Mazzocca 6, T.S. Meehan 9, E. Montaudon 11, F. Nem