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A new method for generating arrayed RNAi screening tools for any organism
EP23750
Poster Title: A new method for generating arrayed RNAi screening tools for any organism
Submitted on 17 Dec 2015
Author(s): Angela Schoolmeesters, Jesse Stombaugh, Megan Basila, Amanda Haas, Annaleen Vermeulen, Melissa L. Kelley, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
Affiliations: Dharmacon (part of GE Healthcare)
This poster was presented at Plant and Animal Genome Conference 2016
Poster Views: 1,606
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Poster Information
Abstract: RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries. For studies in alternate species, siRNAs needed to be custom synthesized at a relatively high cost and very long manufacturing time. To improve the accessibility of these large screening libraries to researchers working in non-human or mouse model systems, we have recently developed a new cost-effective enzymatic manufacturing method to generate large collections of siRNAs, called Dharmacon™ Zoonome™ siRNAs (z-siRNAs). Like other well-established siRNA product lines, they are designed using the proprietary SMARTselection design algorithm for potent and specific gene expression knockdown, but are manufactured on-demand using a novel enzymatic method. Now available are customized arrayed collections of pools of four z-siRNAs to each gene target for screening hundreds or thousands of genes for any organism with an annotated genome. Here we show successful gene knockdown in Cricetulus griseus (Chinese hamster) and Bos taurus (bovine) cell lines using Zoonome siRNAs. With this new availability of large screening libraries, the power of unbiased functional screening in nearly any species can accelerate findings important to animal health, food safety, and infectious disease. Summary: A novel enzymatic production method for on-demand siRNA libraries to support RNAi screening in non-standard species.Report abuse »
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