Posters
« Back
A new method for generating arrayed RNAi screening tools for any organism
EP26577
Poster Title: A new method for generating arrayed RNAi screening tools for any organism
Submitted on 25 Oct 2017
Author(s): Jesse Stombaugh, Megan Basila, Amanda Haas, Annaleen Vermeulen, Melissa L. Kelley, Anja van Brabant Smith
Affiliations: Dharmacon part of Horizon Discovery Group
This poster was presented at GDI conference 2017
Poster Views: 489
View poster »


Poster Information
Abstract: For studies in alternate species, siRNAs needed to be custom synthesized at a relatively high cost and very long manufacturing time. To improve the accessibility of these large screening libraries to researchers working in non-human or mouse model systems, we have recently developed a new cost-effective enzymatic manufacturing method to generate large collections of siRNAs, called Dharmacon™ Zoonome™ siRNAs (z-siRNAs). Like other well-established siRNA product lines, they are designed using the proprietary SMARTselection design algorithm for potent and specific gene expression knockdown, but are manufactured on-demand using a novel enzymatic method. Now available are customized arrayed collections of pools of four z-siRNAs to each gene target for screening hundreds or thousands of genes for any organism with an annotated genome. Here we show successful gene knockdown in Cricetulus griseus (Chinese hamster) and Bos taurus (bovine) cell lines using Zoonome siRNAs. With this new availability of large screening libraries, the power of unbiased functional screening in nearly any species can accelerate findings important to animal health, food safety, and infectious disease. Summary: RNA interference (RNAi) using small interfering RNAs (siRNAs) is an important technology for down-regulation of gene expression and a powerful tool to study cellular processes and pathways. Previously, large collections of siRNAs were available only for traditional experimental model systems, such as human and mouse, and predominantly provided as chemically synthesized libraries.References: Report abuse »
Creative Commons

Related Posters


Multiplex miRNA Profiling for Biomarker Discovery and Verification Studies Using the FirePlex® Platform
M. Tackett, B. Heinrich, I. Diwan, G. Tejada, C. Rafferty, E. Atabakhsh, and D. Pregibon

Platinum™ SuperFi™ DNA Polymerase for the highest success in PCR
Rasa Sukackaitė, Martyna Simutytė, Skaistė Valinskytė, Laurynas Vanagas, Karolis Matjošaitis, Renata Rimšelienė, Remigijus Skirgaila.

DNA-free Platinum Taq DNA polymerase for reliable microbiome studies
Kęstutis Bargaila, Vytautas Budrys, Andrius Krasauskas, Dovilė Lisauskienė, Milda Romeikaitė, Sonata Jurėnaitė-Urbanavičienė,Ramunė Leipuvienė and Juozas Šiurkus

The Good, the Bad and the Ugly: Selective single cell isolation
Sandra Lubos1,2, Nils Körber1, Heide Marie Resch1, Iris Augustin2, Stefan Niehren1

Oral Soft Tissue Lumps and Bumps
Dr. Amal Hassan Zakarya, Sarah Ahmed Mohamed, Noura Mohamed Kamal, Heba Ahmed Saleh