« Back
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
Poster Title: A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
Submitted on 05 Jun 2017
Author(s): Christian Berchtold 1, Guenter Boehm 2, Renée Falsia 2, Thomas Preiswerk 2, Götz Schlotterbeck 1
Affiliations: 1 University of Applied Sciences and Arts Northwestern Switzerland FHNW, Institute for Chemistry and Bioanalytics , Gründenstrasse 40, 4132 Muttenz, Switzerland; 2 CTC Analytics AG; Industriestrasse 20, 4222 Zwingen, Switzerland
This poster was presented at ASMS 2017
Poster Views: 1,153
View poster »

Poster Information
Abstract: - The new LCMS-P 80 Tool is capable of pipetting whole blood with normal hematocrit levels (30-50%) directly from primary tubes with good accuracy and precision, applying one average correction factor. For high hematocrit levels a different correction factor should be applied.

- Wash procedures were established to minimize carryover of analytes. A procedure to minimize the deposition of blood on system surfaces proofed successful.

- The system consisting of a commercial PAL RTC autosampler, connected to an Agilent LC-MS system can perform the complete sample prep and LC-MS analysis for whole blood samples from primary tube. The performance of the system was tested with several typical compounds in therapeutically relevant concentrations.

- Future work is aimed at evaluating the capability of the sensor to reliably detect certain error conditions.
Summary: Automated sample preparation reduces the costs per sample and minimizes sample handling errors. Usually expensive and highly specialized pipetting robots are used. However, most of these systems are not designed with a direct interface for LC-MS applications. In addition common pipetting systems are not optimized for smaller scale sample series. Here we present a new tool for liquid handling of whole blood samples and direct sample injection.
References: [1] Linder et al.; Bioanalysis (2015) 1(16) 2013-2039
[2] Kearney et al.; BMJ. (2006) 332(7553):1302-8
[3] Shah et al.; Nutrition Journal (2011) 10:4
Report abuse »
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters

MALDI Imaging Mass Spectrometry for the study of cardiovascular pathology
Takashi Nirasawa1, Megumi Terada2,3, Hiroko Namba2, Nobuto Kakuda2, Patrick Bruneval3, Hatsue Ishibashi-Ueda4 and Masaya Ikegawa1

Distinct deposition of amyloid-bspecies in brains with Alzheimer’s disease pathology visualized with MALDI imaging mass spectrometry
Nobuto Kakuda1, Tomohiro Miyasaka1, Takashi Nirasawa2, Shigeo Murayama3, Yasuo Ihara1 and Masaya Ikegawa1

Routine Analysis of Host Cell Proteins in Antibody Preparations using PASEF
Stuart Pengelley1, Guillaume Tremintin², Waltraud Evers1, Detlev Suckau1

A Novel Method For Discovery of Peripheral Blood Biomarkers in Idiopathic Pulmonary Fibrosis Using Extensive Depletion and TMTcalibratorTM Tissue-Enhanced Plasma Proteomics
I. Pike1, M. Bremang1, P.J. Wolters 2, R. Gaster3, S. Turner3, M. Decaris3

Salamanca Grosso, G.; Osorio Tangafarife, M.P.