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A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
A New Tool for the Automated Sample Preparation of Whole Blood Samples by LC-MS using a Commercial Autosampler
Submitted on 05 Jun 2017

Christian Berchtold 1, Guenter Boehm 2, Renée Falsia 2, Thomas Preiswerk 2, Götz Schlotterbeck 1
1 University of Applied Sciences and Arts Northwestern Switzerland FHNW, Institute for Chemistry and Bioanalytics , Gründenstrasse 40, 4132 Muttenz, Switzerland; 2 CTC Analytics AG; Industriestrasse 20, 4222 Zwingen, Switzerland
This poster was presented at ASMS 2017
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Poster Abstract
- The new LCMS-P 80 Tool is capable of pipetting whole blood with normal hematocrit levels (30-50%) directly from primary tubes with good accuracy and precision, applying one average correction factor. For high hematocrit levels a different correction factor should be applied.

- Wash procedures were established to minimize carryover of analytes. A procedure to minimize the deposition of blood on system surfaces proofed successful.

- The system consisting of a commercial PAL RTC autosampler, connected to an Agilent LC-MS system can perform the complete sample prep and LC-MS analysis for whole blood samples from primary tube. The performance of the system was tested with several typical compounds in therapeutically relevant concentrations.

- Future work is aimed at evaluating the capability of the sensor to reliably detect certain error conditions.

[1] Linder et al.; Bioanalysis (2015) 1(16) 2013-2039
[2] Kearney et al.; BMJ. (2006) 332(7553):1302-8
[3] Shah et al.; Nutrition Journal (2011) 10:4
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