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A Novel Enzymatic Assay for Determination of Phosphatidylinositol in Biological Samples
Poster Title: A Novel Enzymatic Assay for Determination of Phosphatidylinositol in Biological Samples
Submitted on 04 Jan 2019
Author(s): Paul Templeton, Kyle C. Schmitt, Grigoriy Tchaga,and Gordon Yan
Affiliations: BioVision Inc
This poster was presented at ASCB 2018
Poster Views: 1,150
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Poster Information
Abstract: Phosphatidylinositol (PI) is a molecule central to both phospholipid
metabolism and eukaryotic signal transduction. This phosphoinositide is a
precursor of the important intracellular second messengers diacylglycerol
(DAG) and inositol triphosphate (IP3). PI and its metabolites are intricately
involved in pathways ranging from cell differentiation and growth to
apoptosis. As metabolic diseases such as diabetes and non-alcoholic
fatty liver disease (NAFLD) become more prominent in our society,
monitoring levels of the various biologically active lipid molecules
becomes ever more critical. Rapid turnaround in detection of certain lipids
is also essential to better understand these diseases, as changes in
phospholipid and phosphoinositide concentrations can reflect
dysregulation and alterations in metabolism. BioVision has developed a
novel microplate-based assay for rapid determination of PI in biological
samples, including serum and plasma, cultured cell lysates and tissue
homogenates. The assay is simple to perform, highly selective and can
detect as little as 15 pmole PI in a reaction. Representing the newest
addition to our existing suite of lipid quantification assay kits, this assay is
available exclusively from BioVision Inc. The phosphatidylinositol assay
(Cat. #K750) can be used in conjunction with our other assay kits that
detect and quantify phospholipids and their metabolites – such as
phosphatidic acid (PA), phosphatidylserine (PS), phosphatidylcholine
(PC), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC),
and cardiolipin (CL) – to gain an understanding of the lipid profile in a
variety of sample types.
Summary: • BioVision’s PicoProbeTM Phosphatidylinositol Assay Kit (K750) is a
high-throughput screening assay for measuring phosphatidylinositol
• The assay provides a safe, sensitive and reproducible method of
measuring phosphatidylinositol in under three hours’ time
• This assay kit can be used with many sample types (cell extracts,
tissue lysates and blood samples such as plasma and serum)
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