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A Reduced Workflow Solution for the Analysis of Gamma-Hydroxybutyrate (GHB) in Human Hair Samples Via an Automated Bead Mill as a Precursor to GCxGC-TOFMS and GC-HRT
EP22856
Poster Title: A Reduced Workflow Solution for the Analysis of Gamma-Hydroxybutyrate (GHB) in Human Hair Samples Via an Automated Bead Mill as a Precursor to GCxGC-TOFMS and GC-HRT
Submitted on 09 Mar 2015
Author(s): Brittany M. Watt, David E. Alonso, Joe Binkley, Jeff Patrick, Frank A. Kero, Victor Vandell, Elena Gairloch, Brad Nolt, Tom Enzweller, Rhys Jones, Lee Williams, Karen S. Scott
Affiliations: MSFS Program Arcadia University, The Center for Forensic Science Research and Education, LECO Corporation, Biotage GB Limited
This poster was presented at Pittcon Conference
Poster Views: 1,374
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Poster Information
Abstract: Gamma-hydroxybutyrate (GHB) is an endogenous compound and is also a drug commonly used by bodybuilders, observed in the club scene, and used in drug-facilitated sexual assaults. GHB has been shown to have an elimination rate of 18 mg/L/h, so it is typically cleared from the blood within approximately 6 hours and from the urine within 12 hours after it is taken orally. Hair samples have proven valuable in detecting exposure to drugs of abuse over extended time periods.

Some of the analytical challenges associated with hair analysis include:
• Complexity of the Hair Matrix
• Chemical Diversity of Compounds in Hair
• Coelution of Analytes (e.g., Urea and GHB)
• Concentration Range of Sample Constituents
• Inappropriate Sample Preparation Methods
• Unsuitable Analysis Protocol
Summary: - The Biotage Bead Ruptor 24 was effective in pre-treating hair samples prior to the extraction procedure.
- The enhanced chromatographic resolution of GCxGC-TOFMS and high resolving power of GC-HRT minimized interferences and facilitated accurate analysis of GHB in hair.
References: Brittany M. Watt, David E. Alonso, Joe Binkley, Jeff Patrick, Frank A. Kero, Victor Vandell, Elena Gairloch, Brad Nolt, Tom Enzweller, Rhys Jones, Lee Williams, Karen S. ScottReport abuse »
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