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A Simple and Sensitive Method to Extract Glycan Profiles from Peptide Mapping Data
EP38451
Poster Title: A Simple and Sensitive Method to Extract Glycan Profiles from Peptide Mapping Data
Submitted on 09 Mar 2022
Author(s): 1 Anja Resemann, 1 Stuart Pengelley, 1 Carsten Baessmann , 2 Michael Greig , 1 Detlev Suckau
Affiliations: 1 Bruker Daltonics GmbH & Co. KG, Bremen, Germany 2 Bruker Daltonics ,
Poster Views: 224
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Poster Information
Abstract: Characterization of therapeutic proteins such as monoclonal antibodies requires a broad range of analyses including the confirmation of the protein sequence as well as the identification of post translational modifications such as glycosylation. A common workflow for sequence confirmation is the peptide mapping approach, in which tryptic peptides are sepa rated by reversed phase chromatography and further analyzed using high resolution MS/MS. Typically, N linked glycans are then analyzed in a subsequent dedicated step after release and reducing end labelling. Here, we describe a new method to determine glycan profiles directly from peptide maps using analytical LC and the new VIP HESI ion source and fast MS/MS spectra acquisition using trapped ion mobility separation in a timsTOF Pro 2 instrument.Summary: Here, we describe a new method to determine glycan profiles directly from peptide maps using analytical LC and the new VIP HESI ion source and fast MS/MS spectra acquisition using trapped ion mobility separation in a timsTOF Pro 2 instrument.References: 1: Hilliard M, Alley WR Jr, McManus CA, Yu YQ, Hallinan S, Gebler J, Rudd PM (2017). mAbs, DOI: 10.1080/19420862.2017.1377381
2: 1889617-lcms 188 glycopeptide analysis in peptide mapping workflow ebook.pdfReport abuse »
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