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A Specific Immunocapture Activity Assay for Matrix Metalloproteinases Using 5-FAM/QXL 520™ FRET Peptide
EP20190
Poster Title: A Specific Immunocapture Activity Assay for Matrix Metalloproteinases Using 5-FAM/QXL 520™ FRET Peptide
Submitted on 19 Dec 2013
Author(s): Yi Tang, Xiuqing Li, Xudong Zhu, Xin Han, and Zhenjun Diwu
Affiliations: AnaSpec
Poster Views: 2,113
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Poster Information
Abstract: Addressing the limitations of previous FRET-based MMP assays, this poster documents the development of immunocapture-based FRET assays that are highly sensitive and specifically detect the activity of a particular MMP in a mixed biological sample. The assay sensitivity can reach picogram amounts of enzyme with a large and linear assay range. The assay can also be used to study the interaction between different MMPs.Summary: Addressing the limitations of previous FRET-based MMP assays, this poster documents the development of immunocapture-based FRET assays that are highly sensitive and specifically detect the activity of a particular MMP in a mixed biological sample. Report abuse »
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