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EP23171
Poster Title: An HTS-Compatible Plate For Highly Miniaturized Cultures Of Primary Human Bronchial Epithelial Cells At Air-Liquid Interface
Submitted on 22 Jun 2015
Author(s): Elizabeth Vu, Eric Sorcher, Bob Lowery, Steve Hayes
Affiliations: BellBrook Labs and UAB Cystic Fibrosis Research Center
This poster was presented at NHLBI
Poster Views: 1,655
Submitted on 22 Jun 2015
Author(s): Elizabeth Vu, Eric Sorcher, Bob Lowery, Steve Hayes
Affiliations: BellBrook Labs and UAB Cystic Fibrosis Research Center
This poster was presented at NHLBI
Poster Views: 1,655
Abstract: Primary human bronchial epithelial cells (HBE) cultured at air liquid interface (ALI) exhibit striking similarity to the in vivo situation, including both tissue architecture and ion channel functionality. Cultures of this type serve as a gold standard for predicting therapeutic activity in airway diseases such as cystic fibrosis. An obstacle to use of HBE-ALI models for high throughput screening (HTS) has been the severely limited supply of tissue samples from patients with unusual CFTR mutations. We have developed a plate for highly miniaturized, arrayed HBE-ALI cultures suitable for use in automated drug screening and mechanistic studies (μALI Plate). Cells cultured on the μALI Plate exhibit hallmarks of HBE biology such as beating cilia, but require less than one tenth the number of cells compared to conventional Transwell filters. Cells grown in this manner can be visualized directly from the apical surface by immunocytochemistry without the requirement to image through a filter support. Primary cells grown on the μALI Plate will be useful for testing therapeutic interventions directed towards patients with uncommon CFTR variants. Among other things, the μALI technology has the potential to facilitate personalized approaches to CF therapy, including drug development.
Summary: Development of a HTS compatible air liquid interface culture plate for primary human bronchial epithelial cell differentiation.
Summary: Development of a HTS compatible air liquid interface culture plate for primary human bronchial epithelial cell differentiation.
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