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An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
EP23516
Poster Title: An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
Submitted on 09 Oct 2015
Author(s): Chris Hosfield, Philip Compton, Luca Fornelli, Paul Thomas, Neil L. Kelleher, Michael Rosenblatt & Marjeta Urh
Affiliations: Promega Corporation
Poster Views: 1,374
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Poster Information
Abstract: Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.Summary: Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.References: Chris Hosfield1, Philip Compton2, Luca Fornelli2, Paul Thomas2, Neil L. Kelleher2, Michael Rosenblatt1 & Marjeta Urh1
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711; 2Proteomics Center of Excellence, Northwestern University, Evanston, IL
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