Posters
« Back
An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
EP23516
Poster Title: An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
Submitted on 09 Oct 2015
Author(s): Chris Hosfield, Philip Compton, Luca Fornelli, Paul Thomas, Neil L. Kelleher, Michael Rosenblatt & Marjeta Urh
Affiliations: Promega Corporation
Poster Views: 1,472
View poster »


Poster Information
Abstract: Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.Summary: Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.References: Chris Hosfield1, Philip Compton2, Luca Fornelli2, Paul Thomas2, Neil L. Kelleher2, Michael Rosenblatt1 & Marjeta Urh1
1Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711; 2Proteomics Center of Excellence, Northwestern University, Evanston, IL
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


2D FT0ICR MS/MS analysis of IgG1
Johanna Paris 1 , Tomos E. Morgan 1 , Yuko P. Y. Lam 1 Christopher A. Wootton 1 Mark André Delsuc 2 , Mark P. Barrow 1 , John O’Hara 3 , and Peter B. O’Connor 1

Diagnostic outcomes of MRE as an investigation for NSS of small bowel disease: Can CTE be a cost-effective alternative in patients above 50 years old?
K ElGendy, E Musgrave, M Twemlow

An audit on compliance with the Ottawa Knee Rule in patients with traumatic knee pain
Qasim Afzaal, Saad SIddiqui, Amit Shah

Bleeding Pseudoaneurysm in uncommon locations: interesting cases from St. Peter's Hospital
Dr Joel James (SHO) Dr Chandni Patel (SpR) & Dr Shirish Prabhudesai (Consultant)

Estimating the Mass of the Milky Way Galaxy
Celeste Artiga, Yilun Ma, Ayla Weitz, Alex Ye (Mentor)