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An Integrated <sup>1</sup>H NMR, GC-MS and HPLC-(ESI/TOF) Based Metabolomics Approach to the Study of Acute Exercise in Human Serum Metabolome
EP20299
Poster Title: An Integrated 1H NMR, GC-MS and HPLC-(ESI/TOF) Based Metabolomics Approach to the Study of Acute Exercise in Human Serum Metabolome
Submitted on 19 Dec 2013
Author(s): Beltran, Antoni; Samino, Sara; Brugnara, Laura; Vinaixa, Maria; Rodríguez, Miguel. A; Murillo, Serafín; Bladé, Cinta1; Correig, Xavier; Novials, Anna
Affiliations: CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)
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Abstract: There is an increasing concern about the different response and effect that acute exercise induces on diabetic people and a novel insight into this effect can be performed using a metabolomic approach. Metabolomic is becoming widely spread used as a new and powerful tool for discerning significant changes at metabolic level. In this study we aimed to perform a non-targeted analysis and to identify metabolic differences arisen after 30 minutes of acute exercise in both young men suffering from Type 1 Diabetes Mellitus (T1DM) and control individuals by using three different analytical platforms. A first overlook using 1H NMR to identify the largest compounds presents in the sample and a second stage using both gas (GC) and liquid (LC) chromatography coupled to mass spectrometry to identify the minority compounds.
Summary: There is an increasing concern about the different response and effect that acute exercise induces on diabetic people and a novel insight into this effect can be performed using a metabolomic approach. Metabolomic is becoming widely spread used as a new and powerful tool for discerning significant changes at metabolic level.
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