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Analysis of Urine SRMs Using Solid-Phase Micro Extraction, Dynamic Headspace, and Liquid Injection with Comprehensive Two-Dimensional Gas Chromatography (GCxGC) High Resolution Time-of-Flight Mass Spectrometry
EP24077
Poster Title: Analysis of Urine SRMs Using Solid-Phase Micro Extraction, Dynamic Headspace, and Liquid Injection with Comprehensive Two-Dimensional Gas Chromatography (GCxGC) High Resolution Time-of-Flight Mass Spectrometry
Submitted on 10 Jun 2016
Author(s): David E. Alonso, Joseph E. Binkley, and Jonathan D. Byer
Affiliations: LECO Corporation
This poster was presented at ISCC/GCxGC 2016
Poster Views: 1,090
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Poster Information
Abstract: Laboratory medicine began thousands of years ago through urine analysis. Urine was a “Divine Fluid and Window to the Body”. During Babylonian, Egyptian, and through Victorian times, urine was the primary diagnostic tool (Uroscopy).

Today urine is still a favored biofluid for medical diagnostic testing (Urinalysis) because large volumes are easily obtained. In addition, urine is relatively free from interfering proteins and lipids, and it tends to “hold” high concentrations of drugs and metabolites over extended periods of time. Modern, routine clinical tests include the determination of specific gravity and measurement of levels of glucose, nitrates, etc.

Objectives
o Evaluate different methods of sample introduction
o Implement the use of enhanced, comprehensive (GCxGC) chromatography for separation of urine compounds
o Use modern-day, high resolution time-of-flight mass spectrometry (HRT) and powerful processing software to quickly and confidently identify compounds in urine
Summary: Enhanced two-dimensional chromatographic resolution, spectral similarity searches of large, well-established databases, and formula determinations using high resolution accurate mass ions increases confidence in metabolite identification.

Derivatization and liquid injections greatly increased the number of compound classes amenable to GC-HRT and GC-HRT 4D analyses.
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