Abstract: In immune libraries, the antibody gene repertoire is derived from the immunoglobulin gene of either spleen B cells or peripheral blood B cells from the immunized animals. In comparison with naïve libraries, immune libraries usually produce antibodies of greater affinity, thus avoiding time-consuming in vitro antibody affinity maturation effort. Besides, immune libraries constructed by phage display technology increase the diversity of antibody compared with the conventional hybridoma technology, thus making it possible to produce monoclonal antibodies from a much broader range of species.Summary: Antibody Development by Immune Phage Display Library Construction and Screening
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