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Automated High-throughput Sample Concentration and Buffer Exchange Platform Enhances Rapid Flow Analysis of Antibody Drug Conjugates by UHR-QTOF
EP38455
Poster Title: Automated High-throughput Sample Concentration and Buffer Exchange Platform Enhances Rapid Flow Analysis of Antibody Drug Conjugates by UHR-QTOF
Submitted on 09 Mar 2022
Author(s): Yun Yang1, Guillaume Tremintin1, Philip D. Compton2, Jared J. Drader2, Sheri Manalili Wheeler2
Affiliations: 1Bruker Scientific LLC., Billerica, MA, USA 2Integrated Protein Technologies, Evanston, IL, USA
Poster Views: 415
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Poster Information
Abstract: Antibody drug conjugates (ADCs) as novel protein drug modalities have made considerable progress through the last decades with ten ADCs approved by FDA to date. The structural complexity of ADCs, as well as formulation in MS incompatible buffers, imposes challenges on analytical method development, especially for in-vivo biotransformation samples, which typically require extensive sample preparation and high sensitivity detection. Here, we take advantage of a microfluidic channel-based platform, SampleStream (Integrated Protein Technologies, Fig 1), that uses a molecular weight cutoff (MWCO) membrane for automated buffer exchange and sample enrichment. The platform could be easily coupled with an ultra-high resolution QTOF instrument for the MS analysis and DAR measurement of intact ADCs under native and denaturing conditions.Summary: Here, we take advantage of a microfluidic channel-based platform, SampleStream (Integrated Protein Technologies, Fig 1), that uses a molecular weight cutoff (MWCO) membrane for automated buffer exchange and sample enrichment. The platform could be easily coupled with an ultra-high resolution QTOF instrument for the MS analysis and DAR measurement of intact ADCs under native and denaturing conditions.Report abuse »
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