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Automated Low Input Pulsed-Field Analysis of Genomic DNA
EP34592
Poster Title: Automated Low Input Pulsed-Field Analysis of Genomic DNA
Submitted on 16 Dec 2020
Author(s): Kyle Luttgeharm
Affiliations: Agilent Technologies
This poster was presented at ISBER Annual Meeting 201
Poster Views: 272
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Poster Information
Abstract: Electrophoretic analysis of genomic DNA extractions prior to long-term storage allows Biobanks to provide critical sizing and integrity information to potential customers. Traditionally these separations have been done using pulsed-field slab gel electrophoresis which requires consumption of nanogram amounts of material and long separation times. The Agilent Femto Pulse system overcomes sample consumption and sizing limitations by combining an optimized optical system capable of detecting femtogram levels of DNA with a powerful pulsed-field power supply allowing for separations of genomic DNA through 165 kb. ProSize data analysis software provides smear analysis for average size determination along with the user defined Genomic Quality Number (GQN) aiding in the objective comparison of samples. Use of the Femto Pulse system allows Biobanks to supply accurate sizing and integrity information to customers without consuming precious sample along with the ability to confirm sample quality prior to shipment to the customer. Summary: Use of the Femto Pulse system allows Biobanks to supply accurate sizing and integrity information to customers without consuming precious sample along with the ability to confirm sample quality prior to shipment to the customer. Report abuse »
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