Posters
« Back
Automated Parallel Derivatization Strategy with Broad Metabolite Coverage Coupled to SWATH/MS Data Acquisition for Qualitative and Quantitative Analysis
EP28761
Automated Parallel Derivatization Strategy with Broad Metabolite Coverage Coupled to SWATH/MS Data Acquisition for Qualitative and Quantitative Analysis
Submitted on 29 Jun 2018

David Ruskic, Maria Fernanda Cifuentes Girard; Renzo Picenoni, Guenter Boehm, Gérard Hopfgartner
University of Geneva Department of Analytical and Inorganic Chemistry Life Sciences Mass Spectrometry Geneva Switzerland; CTC Analytics AG Zwingen Switzerland
This poster was presented at ASMS 2018
Poster Views: 572
View poster »
Poster Abstract
Introduction
Diversity in metabolite polarity and electrospray ionization efficiency challenge the development of a single method. Chemical derivatization can significantly improve chromatographic retention time and MS response. We propose a workflow including an automated fast parallel derivatization of amines, phenols, aldehydes, alcohols and ketones followed by QUAL/QUANT SWATH/MS analysis for broad metabolite coverage.

Methods
Dansyl-chloride (DanCl), dansyl-hydrazine (Dan-N2H3) and their 13C label analogs were used as derivatization agents. Samples (49 analyte mix and urine) were derivatized with 12C reagents while standards were derivatized with 13C labeled regents using PAL RTC autosampler (CTC Analytics). The two fractions were mixed and injected onto a column-swtiching LC system. MS acquisition was performed on a TTOF6600 (Sciex) using SWATH acquisition.

Results
Polar metabolites are converted to more hydrophobic products (DanCl and Dan-N2H3 derivatives), enabling them to be separated on reverse phase liquid chromatography. The presence of a basic p-amino group on both reagents increase the electrospray response factor by a factor of 10 to 200. Primary alcohols, phenols, primary and secondary amines are derivatized with DanCl and Dan-N2H3 transforms ketones and aldehydes to hydrazones. The automated parallel derivatization enables reproducible derivatizations in a single workflow including light labeling of sample and heavy 13C labeling of metabolite mix in 15 minutes during the LC analysis of the previous sample. Collision induced dissociation generates fragments specific for the analyte, and for the light and heavy derivatized analytes and for tags (XIC m/z=171 and m/z=173). LC-SWATH/MS which collects all precursors and all fragments allowed the screening and the relative and absolute quantification (n=45) of metabolites in urine.

Conclusion
Labelled reagents enable to generate adequate standards for amines, phenols, aldehydes, alcohols and ketones. Automation was found to be key for reproduciblility and is performed on-line prior LC analysis. In a batch, sample preparation and sample analysis are overlaid resulting in a significant gain of time. Dansyl-chloride and dansyl-hydrazine were found to be ideal for light/heavy labeling, improved LC retention, improved MS response and MS/MS tag

D. Siegel, A.C. Meinema, H. Permentier, G. Hopfgartner, R. Bischoff, Anal Chem, 86 (2014) 5089-5100
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


NEW INSULAR RED PROPOLIS FROM COLOMBIA: BOTANICAL ORIGIN, BIOLOGICAL AND CHEMICAL MARKERS
Salamanca Grosso, G.; Osorio Tangafarife, M.P.

Caracterización de propóleo rojo de la zona insular de San Andrés, Colombia, mediante resonancia magnética nuclear protónica
Guillermo Salamanca Grosso; Monica Patricia Osorio Tangarife; David Fernando García Mendez; Julien Wist

Unique Users-Initiated Biotransformation Reactions for Analysis of Xenobiotic Metabolism
Richard Lee, Rytis Kubilius, Vitaly Lashin, Alexandr Sakharov, Anne Marie Smith

Automated Deformulation of LC/MS and GC/MS Data through Database Searching
Anne Marie Smith, Richard Lee, Artsiom Piatrouski, Andrey Paramonov, Vitaly Lashin

Screening of Common Spices Against Multi-Drug Resistant Mycobacterium tuberculosis
Swati Singh1, Arpita Batta1,2,  Maria Kulsoom1, T. N. Dhole2, B. N. Mishra1, Vineeta Singh1