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Beyond Sequencing CRISPR Gene Editing Assessment and Quality Control Using Next-Generation Sequencing
EP40012
Poster Title: Beyond Sequencing CRISPR Gene Editing Assessment and Quality Control Using Next-Generation Sequencing
Submitted on 07 Mar 2023
Author(s): CD Genomics
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Poster Views: 83
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Poster Information
Abstract: Sequencing-based genome editing validation can detect whether alleles of a gene have been edited correctly, identify insertion and deletion sites at the whole-genome level, and screen and identify monoclonal cell lines. In addition,it is an excellent way to study the off-target effects of CRISPR gene editing. NGS is a massively parallel sequencing strategy that allows for higher throughput. It is widely used for CRISPR library screening, genome editing target validation, and off-target analysis because it is highly sensitive to identifying low-frequency mutations in cell populations.Summary: NGS is a massively parallel sequencing strategy that allows for higher throughput. It is widely used for CRISPR library screening, genome editing target validation, and off-target analysis because it is highly sensitive to identifying low-frequency mutations in cell populations.References: https://www.cd-genomics.com/crispr-screen-sequencing.htmlReport abuse »
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