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Blinded Comparison of an <i>in vitro</i> Diagnostic NGS Assay with Sanger Sequencing for HIV Genotypic Resistance Testing.
EP26326
Poster Title: Blinded Comparison of an in vitro Diagnostic NGS Assay with Sanger Sequencing for HIV Genotypic Resistance Testing.
Submitted on 17 Aug 2017
Author(s): V Varghese1, Pramila Ariyaratne2, PL Tzou1, Elian Rakhmanaliev2, Lee Charlie2, Villy Caroline2, Yee Mei Qi2, Tan Kevin2, BA Pinsky1, Gerd Michel2, RW Shafer1
Affiliations: 1Stanford University School of Medicine, Stanford; 2Vela Diagnostics, Singapore Science Park II, Singapore
This poster was presented at ASM Clinical Virology Symposium, May 7-10, 2017, Savannah, GA, USA
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Poster Information
Abstract: Next generation sequencing (NGS) platforms are increasingly used for HIV genotypic resistance testing in research settings. However, there are few studies in which an in vitro diagnostic NGS product has been blindly compared with standard genotypic resistance testing (SGRT) using dideoxynucleotide Sanger sequencing.
We identified cryopreserved samples that had previously undergone SGRT, and were found to have HIV drug-resistance mutations (DRMs), for a blinded comparison with the the Vela Diagnostics Sentosa SQ HIV Genotyping NGS Assay.
We determined the extent of concordance between SGRT and NGS for the identification of amino acid mutations, drug-resistance mutations (DRMs), and predicted levels of reduced susceptibility to the most commonly used antiretroviral (ARV) drugs.
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