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Bringing Physiological Content to OMICS Studies by Optimizing the Pixel to Chromatogram Connection through SpatialOMx
EP38427
Poster Title: Bringing Physiological Content to OMICS Studies by Optimizing the Pixel to Chromatogram Connection through SpatialOMx
Submitted on 08 Mar 2022
Author(s): Corinna Henkel1, Signe Frost Frederiksen, Nagarjuna Nagaraj1, Sven Meyer1, Tobias Boskamp1, Janine Beckmann2, Janina Oetjen1, Dennis Jakob2, Michael L. Easterling3
Affiliations: 1. Bruker Daltonics GmbH & Co. KG, Bremen, Germany; 2. Max Planck Institute for Marine Microbiology, Bremen, Germany; 3. Bruker Daltonics Inc., Billerica, MA, USA
Poster Views: 189
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Poster Information
Abstract: SpatialOMx® on the timsTOF fleX provides high ID-rates from small sample amounts to combine regiospecificity from MALDI Imaging with PASEF empowered X-Omics. Here we present the SpatialOMx workflow in combination with
4D-Proteomics™. Aim of this study was to find the optimal experimental setup to perform SpatialOMx using MALDI Imaging in combination with Proteomics from the same tissue section. Small tissue pieces were excised from
sections using laser microdissection (LMD) and two different types of slides (IntelliSlides™ or PEN-slides) were compared with regards to their performance in the Omics experiment. Additionally, different staining procedures were
compared to examine their compatibility with the follow-up proteomics experiments.
Summary: Here we present the SpatialOMx workflow in combination with
4D-Proteomics™. Aim of this study was to find the optimal experimental setup to perform SpatialOMx using MALDI Imaging in combination with Proteomics from the same tissue section.
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