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Cas9 Stable Cell Lines
Poster Title: Cas9 Stable Cell Lines
Submitted on 31 Dec 2021
Author(s): Mia Chou
Affiliations: Creative Biogene
Poster Views: 336
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Poster Information
Abstract: CRISPR/Cas9 system used for genome engineering in molecular biology is composed of two components: sgRNA which is a combination of original trRNA and crRNA, and Cas9 endonuclease. By delivering Cas9 and sgRNA into a cell, Cas9 is targeted to a given locus based on sequence complementary between sgRNA and cell genome, and further induces a double-strand break (DSB). Summary: Creative Biogene has developed a series of Cas9 expressing stable cell lines. Cas9 encoding gene is stably integrated into either random site, or AAVS1 safe harbor site of the human genome or ROSA26 safe harbor site of the mouse genome. Apart from HEK293 and HeLa cells, we have achieved Cas9 stable expression in several types of cancer cells which makes it convenient for scientists to study related gene functions of certain cancers.References: abuse »
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