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EP39736
Abstract: Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology utilizes isobaric reagents to label the primary amines of peptides and proteins. (TMT) can be used to identify and quantify proteins in different kinds of samples. Each TMT tagging reagent is composed of an amine-reactive NHS-ester group, a spacer arm and an MS/MS reporter. And the isobaric tagging reagent within a set has the same nominal parent (precursor) mass. The stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) technique performs protein labeling by adding light, medium, or heavy stable isotope-labeled essential amino acids (lysine and arginine) to cell culture media. Summary: Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) technology utilizes isobaric reagents to label the primary amines of peptides and proteins. (TMT) can be used to identify and quantify proteins in different kinds of samples. References: https://www.creative-proteomics.com/services/itraq-based-proteomics-analysis.htm
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