Posters
« Back
Characterizing GPCR Activation Using Automated Live Cell Imaging
EP26130
Characterizing GPCR Activation Using Automated Live Cell Imaging
Submitted on 11 Jul 2017

Joe Clayton and Peter Banks
BioTek Instruments, Inc. Winooski, VT USA
Poster Views: 339
View poster »
Poster Abstract
G protein coupled receptor (GPCR)-mediated pathways are critical for cells to respond to intercellular and environmental cues, and are a major focus of drug discovery efforts, particularly for cancer treatment. The molecules that activate GPCRs, and the resulting signaling cascades triggered by associated G proteins, are diverse. Fluorescent dyes and biosensors can be used to monitor changes in second messenger levels, including Ca2+ and cyclic AMP (cAMP), in response to GPCR activation. Here we describe a live cell imaging based approach to detect GPCR activation using the Lionheart™ FX Automated Live Cell Imager and Gen5™ Microplate Reader and Imager Software. This method provides a large assay window and improved sensitivity over methods relying on total fluorescence intensity measurements. Dual in-line dispense tips enable addition of GPCR agonists with continuous monitoring of cellular response. Additionally, an image capture rate of up 20 frames per second enables characterization of rapid GPCR kinetics.

1. Together, the Lionheart FX Automated Live Cell Imager and Montana Molecular biosensors provide a versatile and robust system for detecting biologically relevant GPCR signaling.
2. Up to 20 fps image capture and dual in-line reagent injectors allow for uninterrupted monitoring of rapid cellular responses including Ca2+ flux and Gs/Gi-dependent regulation of cAMP production.
3. Imaging-based approach to detecting GPCR activation enables detailed characterization of single cell kinetic profiles and percent responder measurements.
4. 96-well format and automated image capture and analysis increases GPCR assay productivity and reproducibility.
Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Tau overexpression leads to uncoupling of mTORC1 signaling in neuronal cells
Chao Ma MS1,2,3, Andrii Kovalenko BS1,2, John Calahatian1,2, Huimin Liang MS1,2, Mani Kallupurackal1,2, Jerry Hunt BS1,2, Kevin Nash PhD1,3, Margaret Fahnestock PhD4, Dave Morgan PhD1,3, Paula Bickford PhD1,3, Daniel Lee PhD1,2

Novel Role of the Innate Immune DNA Sensor IFI16 (Interferon Gamma Inducible Protein 16) as a Major Epigenetic Modulator During KSHV Infection and Lytic Reactivation
Arunava Roy, Anandita Ghosh, Bala Chandran

HSF-1 is a Regulator of miRNA Expression in Caenorhabditis elegans
Alana Snyder; Jessica Brunquell, Ph.D.; Feng Cheng, Ph.D.; Sandy Westerheide, Ph.D.

P450 INDUCTION IN CRYOPRESERVED HEPATOCYTES FROM PXR AND CAR NUCLEAR RECEPTOR KNOCK-OUT RATS
Kevin P. Forbes1 , Kirsten Amaral2 and Albert P. Li2

The added value of traditional bulk sample measurements in single cell RT-qPCR experiments
Lukas Valihrach1, David Dzamba2, Peter Androvic1, Miroslava Anderova2, Mikael Kubista1,3