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Charge variant analysis of Cetuximab with Chip Based CZE MS
EP38446
Poster Title: Charge variant analysis of Cetuximab with Chip Based CZE MS
Submitted on 09 Mar 2022
Author(s): Yue Ju 1 , Guillaume Tremintin 1 , Aditya Kulkarni 2
Affiliations: 1 Bruker Scientific LLC., San Jose, CA, US 2 908 Devices, Boston, MA, US
Poster Views: 410
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Poster Information
Abstract: Charge variants related to glycosylation, C terminal processing, deamidation or glycation often occur in mAb based biotherapeutics. Routine charge variant analysis is conducted throughout manufacturing to evaluate quality attributes that could affect the final drug quality, safety, and potency. Monoclonal antibody Cetuximab has four N glycan sites with two sites on each heavy chain located in the Fc and CH 1 domain Capillary zone electrophoresis (CZE) coupled with high resolution mass spectrometry provides a powerful MS hyphenated alternative to identify charge variants present in complex biopharmaceuticals. ZipChip is a microfluidic device integrating CZE with electrospray ionization. In this work, ZipChip is coupled to Bruker’s Ultra High Resolution QTOF maXis II mass spectrometer for deep profiling of Cetuximab charge variants.
Summary: In this work, ZipChip is coupled to Bruker’s Ultra High Resolution QTOF maXis II mass spectrometer for deep profiling of Cetuximab charge variants.Report abuse »
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