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Chemical Variant of 7-deaza-dGTP for Improved CG-rich PCR Amplification
EP20079
Poster Title: Chemical Variant of 7-deaza-dGTP for Improved CG-rich PCR Amplification
Submitted on 19 Dec 2013
Author(s): E. H. Ashrafi, S. Shore, T. Le, V. Timoshchuk, N. Paul, R. Hogrefe, G. Zon, I. Koukhareva, A. Lebedev
Affiliations: TriLink BioTechnologies, Inc.
Poster Views: 1,832
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Poster Information
Abstract: PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its wide use, certain GC-rich regions of DNA, such as mycobacterial disease targets, still remain a challenge for amplification. Sequences high in GC content are associated with the formation of secondary structure, which prevents adequate strand separation and DNA polymerase amplification. As a consequence, mispriming is prominent, complicating specific product formation.Summary: PCR amplification of nucleic acids is a fundamental technique used in many molecular biology laboratories. Despite its wide use, certain GC-rich regions of DNA, such as mycobacterial disease targets, still remain a challenge for amplification. Report abuse »
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