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Chemopreventive Effect of Mentha Piperita on Dimethylbenz[a]anthracene and Formaldehyde-induced Tongue Carcinogenesis in Mice (Histological and Immunohistochemical study)
EP31290
Poster Title: Chemopreventive Effect of Mentha Piperita on Dimethylbenz[a]anthracene and Formaldehyde-induced Tongue Carcinogenesis in Mice (Histological and Immunohistochemical study)
Submitted on 07 Feb 2020
Author(s): Rehab F. Kasem1, Radwa H. Hegazy2,*, Mona A. A. Arafa3, Mona M. AbdelMohsen4
Affiliations: 1Professor of Oral and Maxillofacial Pathology, Faculty of Dentistry, Cairo University and the Head of the Oral Pathology Department, Faculty of Dentistry, October university of Modern Science and Arts (MSA). 2Professor of Oral Biology, Faculty of Dentistry, Cairo University. 3Faculty of Medicine for Girls, Department of Anatomy, Al-Azhar University. 4Phytochemistry Department, National Research Center in Egypt
This poster was presented at the second annual scientific day of the oral and maxillofacial pathology department, Cairo University
Poster Views: 322
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Poster Information
Abstract: OBJECTIVE: Cancer chemoprevention is defined as the
use of chemicals or dietary components to block, inhibit,
or reverse the development of cancer in normal or preneoplastic
tissue. Mentha extract (ME) has antioxidant and
antiperoxidant properties. This study was held to investigate
the protective and anticancer effect of Mentha leaves
aqueous extract on oral epithelium of mice tongues.
DESIGN: A total of 80 Egyptian albino mice were divided
into three groups. Group I served as control (not
subjected to any kind of treatment), and groups II and
III were subjected to two-stage chemical carcinogenesis
through topical application of dimethylbenz[a]anthracene
(DMBA) followed by formaldehyde on dorsal and
ventral surfaces of tongues for 9 weeks. Mentha leaves
extract was administrated to group III at the same time
of cancer induction. Histological changes were assessed in
H&E sections at 3-week intervals. The anticarcinogenic
effect of Mentha piperita was tested using immunostain
with anticaspase antibody.
RESULTS: The oral administration of ME reduced the
appearance of dysplastic cellular changes with 61% and
inhibited tumor incidence with 100%. Group I showed
moderate-to-strong cytoplasmic caspase expression. At
6-week interval, group II showed weak-to-moderate caspase
expression, while sections from group III showed moderate-
to-strong caspase expression. High significant statistical
difference in the total score of caspase 3 expression was
found between specimens obtained from animals sacrificed
at 6 weeks in groups I, II, and III (P = 0.001**).
CONCLUSION: Our study demonstrated that Mentha
piperita has inhibited the initiation and promotion of oral
dysplastic lesions.
Summary: Mentha extract (ME) has antioxidant and
antiperoxidant properties. This study was held to investigate
the protective and anticancer effect of Mentha leaves
aqueous extract on oral epithelium of mice tongues.
References: 1. Taso AS, Kim ES, Hong WK. Chemoprevention of Cancer.
CA Cancer J Clin 2004; 54: 150–80.
2. Tanaka T. Cancer chemoprevention by natural products. Oncol
Rep 1994; 1: 1139–55.
3. Morse EC, Stoner G. Cancer chemoprevention principles and
prospects. Carcinogenesis 1996; 14: 1737–46.
4. Pezzuto JM. Plant-derived anticancer agents. Biochem Pharmacol
1997; 53: 121–33.
5. Lea MA. Organosulfur compounds and cancer. Adv Exp Med
Biol 1996; 401: 147–54.
6. World Health Organization, Traditional medicine. Available
at: http://www.who.Int/mediacentre/factsheets/fs134/en/
(accessed on 6 April 2010).
7. Phillipson JD. Phytochemistry and medicinal plants. Phytochemistry
2001; 56: 237–43.
8. Kamatou GPP, Makunga NP, Ramogola WPN, Viljoen AM.
South African Salvia species: a review of biological activities
and phytochemistry. J Ethnopharmacol 2008; 119: 664–72.
9. Nair B. Final report on the safety assessment of Mentha piperita
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