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Choose the Best 16S/18S/ITS Sequencing Method for Your Research
EP37815
Poster Title: Choose the Best 16S/18S/ITS Sequencing Method for Your Research
Submitted on 02 Nov 2021
Author(s): CD Genomics
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Poster Views: 78
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Poster Information
Abstract: CCS allows the polymerase to replicate the circularized strand repeatedly, resulting in a single lengthy read with randomly scattered mistakes. Microbiome studies using PacBio CCS mode will give high-fidelity species-level data. Summary: PacBio's long-read sequencing technique was previously plagued by significant error rates per base. Instead, the PacBio circular consensus sequencing (CCS) method can provide extremely accurate (99.8 percent) long reads with an average length of 13.5 kb, in addition to full-length 16S/18S/ITS reads. CCS allows the polymerase to replicate the circularized strand repeatedly, resulting in a single lengthy read with randomly scattered mistakes. Microbiome studies using PacBio CCS mode will give highReferences: https://www.cd-genomics.com/16S-18S-ITS-Amplicon-Sequencing.htmlReport abuse »
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