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Comparative evaluation of a new processing pipeline for PASEF Label Free Quantification analysis.
EP38470
Poster Title: Comparative evaluation of a new processing pipeline for PASEF Label Free Quantification analysis.
Submitted on 09 Mar 2022
Author(s): Patrick Emery 1 , Manuel Chapelle 2 , Pierre Olivier Schmit 2 and Aurélie Même 2
Affiliations: 1 Matrix Science ltd, London, UK. 2 Bruker France S.A., 67160 Wissembourg , France
Poster Views: 388
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Poster Information
Abstract: Parallel Accumulation Serial Fragmentation (PASEF) data acquisition strategies have changed the way proteomics data are recorded in many different way: on top of the combined speed and sensitivity increase, the additional separation of target ions in the ion mobility dimension as well as the systematic measurement of their collisional cross section (CCS) has dramatically increased the data files information content. The recently introduced 4 D proteomics approaches are making an extensive use of the ion mobility separation and of the CCS information to increase the identification reliability, the data completeness, and the quantitation accuracy. Here, we have evaluated the performances of a newly introduced processing pipeline and compared it to the established MaxQuant and Peaks Studio platformsSummary: Here, we have evaluated the performances of a newly introduced processing pipeline and compared it to the established MaxQuant and Peaks Studio platformsReport abuse »
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