Posters
« Back
Comparison of hepatocytes in monolayer and RAFT™ 3D Cell Culture System
EP29425
Comparison of hepatocytes in monolayer and RAFT™ 3D Cell Culture System
Submitted on 29 Nov 2018

Therese Willstaedt, Maureen Bunger, Lubna Hussain, and Theresa D’Souza
Lonza
This poster was presented at SOT 2018
Poster Views: 120
View poster »
Poster Abstract
Hepatocytes comprise 70 – 85% of the liver’s mass and are the major cell type involved in detoxification. In the pharmaceutical industry there is an increasing demand for hepatocyte-based models which mimic human in vivo toxicity. For in-vitro screening, the current gold standard for drug metabolism and toxicity consists of hepatocytes grown is a traditional two dimensional (2D) culture system. However, it has been reported that although primary hepatocytes briefly maintain some functional capabilities in monolayer culture, such as the production of drug-metabolizing enzymes, they rapidly lose their morphology along with the majority of liver-specific functions. We compared cell viability, cell morphology and ZO-1 expression of primary rat and human hepatocytes grown as a monolayer on collagen and sandwiched by Matrigel with that of cells cultured within collagen hydrogel in three dimensions using RAFT™ Culture System. To determine the effect of growing hepatocytes in RAFT™ Culture compared to sandwich culture, we treated the human hepatocytes in both models with inducers like Omeprazole, Phenobarbitol and Rifampycin and examined the metabolic cytochrome P450 (CYP) capabilities. For hepatocytes in RAFT™ Culture System the response was both stronger and more stable over 17 days in culture compared to the sandwich cultures. Improved stability of P450s by culturing hepatocytes in 3 Dimensions could enable long-term and repeat dosing for toxicity analysis using primary human hepatocytes.

Report abuse »
Questions
Ask the author a question about this poster.
Ask a Question »

Creative Commons

Related Posters


Investigation of the graphene-on-silicon-carbide and CVD graphene as a basis for bioimpedance sensor applications
Natalja Sleptsuk (a), Raul Land (a), Jana Toompuu (a), Alexander Lebedev (b), Valery Davydov (b), Ilya Eliseyev (b), Evgenia Kalinina (b), Oleg Korolkov (a) and Toomas Rang (a)

Unique Users-Initiated Biotransformation Reactions for Analysis of Xenobiotic Metabolism
Richard Lee, Rytis Kubilius, Vitaly Lashin, Alexandr Sakharov, Anne Marie Smith

New BIONET Compounds for CNS Diseases
Steve Brough1, Andrew Lowerson1 and Nikolay T. Tzvetkov2

High-throughput Carbonic Anhydrase Activity and Inhibitor Screening Assays
Suratna Hazra, Ph.D., Grigoriy Tchaga, Ph.D., and Gordon Yan, Ph.D.

A High-Throughput Assay to Measure Phosphoenolpyruvate Carboxykinase (PEPCK) Activity in Biological Samples
Shunan Li, Ph.D., Keith Cheung, Ph.D. Grigoriy Tchaga, Ph.D. Jianping Xu, Ph.D. and Gordon Yan, Ph.D. Acknowledments: Kyle Schmitt, Ph.D • Tanvi Gangakhedkar • Gustavo Chavarria, Ph.D. • Kwang Kim, Ph.D. • Robert Stephenson, Ph.D. • Hong Qu, Ph.D.