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Comparison Of Three Label Based Quantification Techniques
EP34763
Poster Title: Comparison Of Three Label Based Quantification Techniques
Submitted on 28 Jan 2021
Author(s): Creative Proteomics
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Poster Views: 183
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Poster Information
Abstract: This infographic introduces three label-based quantification techniques: iTRAQ, TMT and SILAC. Belong to in vitro labeling quantification, iTRAQ and TMT have a similar principle. iTRAQ uses two main reagents: 4-plex and 8-plex, while there are up to 11 tags in TMT. Both techniques can label almost any peptide or protein samples. SILAC is a in vivo labeling method for quantitative proteomics. Protein labeling can be performed by adding light, medium or heavy isotope labeled essential amino acids (lysine and arginine) to the cell culture medium.Summary: TMT and SILAC. Belong to in vitro labeling quantification, iTRAQ and TMT have a similar principle. iTRAQ uses two main reagents: 4-plex and 8-plex, while there are up to 11 tags in TMT. Both techniques can label almost any peptide or protein samples. SILAC is a in vivo labeling method for quantitative proteomics. Protein labeling can be performed by adding light, medium or heavy isotope labeled essential amino acids (lysine and arginine) to the cell culture medium.References: https://www.creative-proteomics.com/services/tmt-based-proteomics-service.htmReport abuse »
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