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Contrasting Patterns of Neutral and Adaptive Genetic Variation of Chilean Blue Mussel (Mytilus chilensis) Due to Local Adaptation and Aquaculture
Poster Title: Contrasting Patterns of Neutral and Adaptive Genetic Variation of Chilean Blue Mussel (Mytilus chilensis) Due to Local Adaptation and Aquaculture
Submitted on 29 Dec 2014
Author(s): Cristian Araneda1 , M. Angelica Larrain2, Benjamin Hecht3, Shawn Narum4
Affiliations: 1Universidad de Chile, Depto. de Produccion Animal, Santiago, Chile. 2Universidad de Chile, Departamento de Ciencia de los Alimentos y Tecnología Química, Santiago, Chile. 3University of Idaho, Hagerman, ID, USA. 4Columbia River Inter-Tribal Fish Commission, Hagerman, ID, USA.
This poster was presented at Plant and Animal Genome XXIII Conference
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Poster Information
Abstract: Like many marine invertebrates, Chilean blue mussel populations have been thought to be panmictic with limited genetic structure due to pelagic larvae. Advances in genotyping-by-sequencing have enabled investigation of adaptive genetic variation that may identify outlier loci that better distinguish populations that have evolved in different environments. We studied 190 mussels sampled in 6 locations from southern Chile (4 from Reloncaví Gulf, one from Chiloe Island and one from Patagonia) with 2,165 SNP loci obtained with RAD-seq. Differentiation among collections with 891 neutral loci was very low (FST = 0.0051). Differentiation among all six collections was much higher with putative outlier loci 58 (FST = 0.1139) and indicated potential for local adaptation. DAPC results with these 58 outlier loci demonstrated that much of the differentiation could be attributed to the three major regions and environments: extreme conditions in Patagonia, inner bay collections influenced by freshwater (Reloncaví area), and outer bay saltwater collection (Chiloe Island). The southern Patagonia collection was most distinct, but additional outlier tests with this collection excluded revealed that adaptive divergence remained between inner and outer bay collections (34 candidate loci, FST= 0.0886). The four locations from the inner bay area of Reloncaví were largely panmictic with all panels of markers, likely due to similar environments and high gene flow from aquaculture activities and low geographic distance. However, our results show that it is possible to separate inner and outer bay populations in the culture area, with individual assignment of 51% to these areas providing the opportunity for traceability of aquaculture production. This study demonstrates how adaptive genetic variation can be utilized for applications related to human propagation and distribution of species with high gene flow.Summary: This study was designed to investigate patterns of neutral and adaptive genetic variation within Chilean blue mussel populations in order to identify a subset of putatively adaptive genetic markers to investigate the population structure and to improve the ability to trace individuals to their geographical origin, especially in the area with strong aquaculture activitiesReferences: Antao T et al. (2008). BMC Bioinformatics 9, 323 .
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