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Crosslinking & Label Transfer Protein Interaction Analysis - Creative Proteomics
Poster Title: Crosslinking & Label Transfer Protein Interaction Analysis - Creative Proteomics
Submitted on 01 Mar 2021
Author(s): Creative Proteomics
Poster Views: 226
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Poster Information
Abstract: The label transfer approach incorporates crosslinking methodology to study protein-protein interactions by labelling proteins that interact with a protein of interest. This method
can be used to discover novel interactions, confirm putative interactions suggested by
other techniques, and investigate protein complexes. In addition, the label transfer
method can detect weak or transient protein interactions that often fail to detect by co-immunoprecipitation, or pull-down method.
Summary: Under physiological condition, most protein-protein interactions are transient, and happen in a very short duration, increasing the difficulties to study them. Crosslinking reagents, or crosslinkers, provide the analytical solution to capture protein-protein complexes by covalently binding them together as they interact and freezing even transient, weak interactions for consequent isolation and characterization.
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