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Cytotoxicity of Environmental Toxins PFOA and Gen X
Poster Title: Cytotoxicity of Environmental Toxins PFOA and Gen X
Submitted on 20 Feb 2020
Author(s): Lauren Zane, Thomas Schultz
Affiliations: Duke University Marine Lab, Duke Cancer Institute, University of California, Berkeley
This poster was presented at Ocean Sciences Meeting 2020
Poster Views: 334
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Poster Information
Abstract: Several PFAS (Poly- and per-FluoroAlkyl Substances) compounds, largely used in the production of Teflon and other materials have demonstrated bioaccumulative, environmentally persistent and carcinogenic properties. In the effort to replace these compounds, shorter chain chemicals hypothesized to be less toxic, such as Gen X, have been utilized in Teflon processing. Gen X while still environmentally persistent, does not bioaccumulate like its predecessor PFOA (perfluorooctanoic acid). Since 2017, concerns over Gen X contamination of the Cape Fear River near Wilmington, North Carolina and the lack of studies on the toxicity of Gen X have driven the need to conduct further studies of the chemical. In this study, NIH3T3 cells were exposed to eight concentrations of PFOA ranging from 0-2.5mM or Gen X ranging from 0-5mM for 24 hours to be analyzed with cell viability assays using cell counts, ATP production, and staining for apoptosis. These assays have demonstrated that Gen X is a less cytotoxic chemical than PFOA.Summary: The presence of PFAS, in drinking water have raised concerns over water quality after chemicals belonging to this family have been detected in drinking water in 43 cities in the US. In particular, the chemical Gen X is of interest because of its long term presence in the Wilmington, NC area. There are very limited toxicity studies concerning the chemical, thus this study seeks to understand the physiochemical and toxicological properties associated with the compound. References: Beekman, M. Evaluation of Substances Used in the Gen X Technology by Chemours, Dordrecht.
Caverly Rae, J. M., et al. “Evaluation of Chronic Toxicity and Carcinogenicity of Ammonium 2,3,3,3-Tetrafluoro-2-(Heptafluoropropoxy)- Propanoate in Sprague–Dawley Rats.” Toxicology Reports, vol. 2, 2015, pp. 939–49.
Gannon, Shawn A., et al. “Absorption, Distribution, Metabolism, Excretion, and Kinetics of
2,3,3,3-Tetrafluoro-2-(Heptafluoropropoxy)Propanoic Acid Ammonium Salt Following a
Single Dose in Rat, Mouse, and Cynomolgus Monkey.” Toxicology, vol. 340, Jan. 2016, pp. 1–9. (Crossref), doi:10.1016/j.tox.2015.12.006. Lau, Christopher, Katherine Anitole, Colette Hodes, David Lai, Andrea Pfahles-Hutchens, and Jennifer Seed, ‘Perfluoroalkyl Acids: A Review of Monitoring and Toxicological Findings’, Toxicological Sciences, 99.2 (2007), 366–94
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