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DC microelectrode array for cell investigation
Poster Title: DC microelectrode array for cell investigation
Submitted on 17 Mar 2015
Author(s): Marisa Rio1 , Sharanya Bola2, Salvatore Girardo3, Richard H. Funk2, Gerald Gerlach1
Affiliations: 1 Institute of Solid State Electronics, Faculty of Electrical and Computer Engineering, Technische Universität Dresden, Germany; 2 Department of Anatomy, University Hospital Carl Gustav Carus, Technische Universität Dresden, Germany; 3 Biotechnology Center, Technische Universität Dresden, Germany
This poster was presented at Lab-on-a-Chip & Microfluidics
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Poster Information
Abstract: The discovery that cells undergo electrotaxis, orient and migrate in a specific direction relatively to electric fields, dates for the late 19th century. Recent studies suggest that electrically sensitive cells lines are the rule and not the exception. The influence of endogenous DC electric fields is now shown to be present in many cell biological phenomena, ranging from cell adhesion and migration, embryonic and tissue development to wound healing.
In the present study, we report the steps towards the assembling of an electrically stimulated microfluidic biochip. In order to mimic the in vivo environment DC electric fields are used. This biochip intends to stimulate and record the response of cells to different DC electric fields. Its main feature involves the replacement of conventional metallic microelectrodes positioned directly beneath the cells surface by channels filled with conductive medium, therefore delaying Faradaic products to attain the cell surface. The biochip architecture is based on multilayer integration over a polycarbonate base plate containing Ag/AgCl electrodes.
The first results showed the biocompatibility of the microfluidic biochip and promise to render further understanding of the cellular interaction with DC electric fields.
Summary: In order to mimic the endogenous dc electric field that cells experience in vivo, a microelectrode array was designed. Preliminary tests showed biocompatibility and promise to render further understanding of the cellular interaction with DC electric fields.References: [1 ] Du Bois-Reymond, E. Vorläufiger Abriss einer Unterschung uber den sogenannten Froschstrom und die electromotorischen Fische.
1843, Ann. Phy. U. Chem. 58, pp. 1 -30.
[2] Galvani L. Aldini, G. Aloysii Galvani: de viribus electrocitatis in motu musculari. 1792, Apud Societatem Typographicam.
[3] McCaig, C. Rajnicek, A.M. Song, B. Zhao, M. Controlling cell behavior electrically: current views and future potential. 2005, Physiol.
Rev. 85, pp. 943-978.
[4] Funk, R. H.W. Monsees, T. Özkucur, N. Electromagnetic effects – From cell biology to medicine. 2009, Progress in histological and
cytochemistry 43, pp. 177-264.
[5] Messerli, M. A. Grahan, D.A. Extracellular Electrical Fields Direct Wound Healing and Regeneration. 2011 , Biol. Bull. 221 , pp. 79-92.
[6] Levin, M. Large-scale biophysics: ion flows and regeneration. 2007, Trends in Cell Biology, pp. 261 -270.
[7] Derix, Jonathan. Biokompatibles Gleichstrom-Mikroelektrodenarray. Dresden : TUD Press, 20
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