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Defining off-target cleavage in a pair of Zinc Finger Nucleases
EP20239
Poster Title: Defining off-target cleavage in a pair of Zinc Finger Nucleases
Submitted on 19 Dec 2013
Author(s): K. Mukherjee, D. Carroll
Affiliations: University of Utah
Poster Views: 1,309
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Poster Information
Abstract: Studies on Zinc Finger Nucleases (ZFN) have shown that they can be toxic in organisms. This is potentially due to ZFN cleavage at multiple off-target sites. In applications of ZFNs in human gene therapy, this off-target cleavage is intolerable. We are attempting to develop a procedure to identify these off-target sites in Drosophila. We can then analyze every new ZFN pair for potential off-target cleavage and select and redesign it to work more efficiently.Summary: This study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs.Report abuse »
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