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Degradation of Artificially Aged Vegetable-Tanned Leather using RP-HPLC and FTIR-ATR
Poster Title: Degradation of Artificially Aged Vegetable-Tanned Leather using RP-HPLC and FTIR-ATR
Submitted on 28 Oct 2019
Author(s): Yadi Hu, Eleni Tziamourani, SC Boyatzis, Jingru Wang, Lvyang Wang, Keyong Tang
Affiliations: School of Materials Science and Engineering,Zhengzhou university,Zhengzhou, Henan, 450001 and University of West Attica, Department of Conservation of Antiquities & Works of Art, Egaleo
This poster was presented at 11th Asia International Conference of Leather Science and Technology (AICLST), hosted by the China Leather Industry Association (CLIA) and organized by Shaanxi University of Science and Technology (SUST) in the city of Xi’an, Shaanxi province, China on the 16th -19th Oct., 2018.
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Poster Information
Abstract: As a collagen-based material, leather is susceptible to such environmental factors as temperature, relative humidity, microbes, light, and so on, especially to the sudden and great changes of these parameters. The amino acids of collagen in leather are mainly glycine, repeated on Gly-X-Y in tripeptides, where the X and Y are generally proline and hydroxyproline respectively. It is well known that degradation occurs in leather, usually accompanying the deterioration of collagen structure. In this paper, Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) and Fourier Transform Infrared-Attenuated Total Reflection (FTIR-ATR) were employed to investigate the effects of temperature and relative humidity on the degradation of modern commercial vegetable-tanned leather and the deterioration of collagen structure by degradation were discussed. The samples were artificial aged at 50°C,40% RH for 0 day, 3 days and 8 days respectively. Results of RP-HPLC indicated that relative concentrations of proline and hydroxyproline decreases after the artificially aging treatment. FTIR-ATR analysis showed that the amide I band of C=O at 1660 cm-1 increases whereas the amide II band at 1539 cm-1 decreases, which was possibly attributed to the new free amino acids or peptides by aging. Meanwhile, the triple helix structure of collagen was destroyed by artificial aging. Therefore, the intensity of peak at 1634 cm-1 which is related to random coil content gradually increases with increasing the aging time. Summary: Leather has been used for centuries for clothing. It is a complex natural collagen-based biological martial constituent of many precious artifacts including armor, boots, etc. However, leather may easily degrade by the effect of temperature, humidity, light, microorganism and pollutants. Therefore, their conservation is determined by the storage conditions. These environmental factors alone or synergistically will lead to different degradation of leather. References: [1] Budrugeac P, Carşote C, Miu L. Application of thermal analysis methods for damage assessment of leather in an old military coat belonging to the History Museum of Braşov-Romania. Journal of Thermal Analysis & Calorimetry, 2017, 127(1):1-8.
[2] Cucos A, Budrugeac P, Miu L. DMA and DSC studies of accelerated aged parchment and vegetable-tanned leather samples. Thermochimica Acta, 2014, 583(14):86-93.
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