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DETERMINATION OF TERBUTHYLAZINE IN URINE OF RATS EXPOSED TO ITS LOW DOSES
EP25683
Poster Title: DETERMINATION OF TERBUTHYLAZINE IN URINE OF RATS EXPOSED TO ITS LOW DOSES
Submitted on 06 Apr 2017
Author(s): Marija Dvoršćak, Gordana Mendaš, Sanja Fingler, Sanja Stipičević,
Affiliations: Institute for Medical Research and Occupational Health, Biochemistry and Organic Analytical Chemistry Unit, Zagreb, Croatia
This poster was presented at 5th Croatian Congress of Toxicology with International Participation, CROTOX2016, Poreč, October 9-12 2016
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Poster Information
Abstract: Pesticides are a major group of chemicals produced to be intentionally released in the environment. Many pesticides are not easily degradable; they persist in soil, leach to groundwater and surface water and contaminate wide environment. Depending on their chemical properties they can enter an organism, bioaccumulate in food chains and consequently influence human health. There is a lot of data concerning acute exposure of people or laboratory animals to pesticides. However, long-term exposure to low concentrations corresponding to real-life scenarios is much less investigated. Exposure to triazine herbicides such as terbuthylazine mainly results in urinary excretion of the parent compound and of its metabolites formed by dealkylation. The aim of this study was to determine mass concentrations of terbuthylazine and its metabolites in rat urine. Animals were exposed to tree different concentrations of terbuthylazine consecutively for 28 days. Terbuthylazine and its metabolites were determined in a 24-hour urine sample collected on the first, 14th and 28th day of treatment. The results will serve to define the markers and biomarkers of exposure. Different extraction procedures were developed for the accumulation of triazine herbicide terbuthylazine and chlorodealkylated metabolites from rat urine: extraction with ethyl-acetate and solid-phase extraction on styrene-divinylbenzene sorbents. These procedures were optimised for a final analysis by high performance liquid chromatography. Mass concentration of terbuthylazine recovered from urine indicates that unchanged terbuthylazine represents only a minor part of adsorbed dose while the majority of terbuthylazine was excreted as dealkylated metabolites.
This work has been supported in full by the Croatian Science Foundation under the project no. 8366.
Summary: Investigation of long-term exposure to low concentrations of terbuthylatrazine corresponding to real-life scenarios. The aim of this study was to determine mass concentrations of terbuthylazine and its metabolites in rat urine of animals exposed to tree different concentrations of terbuthylazine consecutively for 28 days. Different extraction procedures were developed and optimised for a final analysis by high performance liquid chromatography.References: Report abuse »
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