Posters
« Back
Development and optimization of a procedure for extracting proteins from fixed archival tissues and assessment of its potential for biomarker discovery in human and veterinary medicine
EP20681
Poster Title: Development and optimization of a procedure for extracting proteins from fixed archival tissues and assessment of its potential for biomarker discovery in human and veterinary medicine
Submitted on 23 Dec 2013
Author(s): Alessandro Tanca, Daniela Pagnozzi, Sergio Uzzau, Maria Filippa Addis
Affiliations: Porto Conte Ricerche
Poster Views: 1,496
View poster »


Poster Information
Abstract: Fixed tissue repositories are an ideal information mine for biomarker discovery, with their vast collections of diseased tissues and associated clinical records describing diagnosis, prognosis, therapy, and outcome. Unfortunately, fixation hampers protein extraction, posing considerable limitations to proteomic investigations. In this scenario, and building on the existing literature, several methods were combined and refined to enable extraction of high-quality, full-length proteins from the fixed specimens, leading to the definition of an optimized protocol with unprecedented performances. In order to test its ability to provide meaningful results in terms of differential disease traits and enable biomarker discovery, the optimized method was applied to sample repositories stored in pathology departments. Specifically, diseased human, canine and sheep tissues were retrieved and subjected to the optimized extraction procedure, followed by established proteomic pipelines consisting of electrophoresis, image analysis, mass spectrometry, and data processing by ontology and pathway analysis. The method enabled gel-based proteomic investigations of archival tissues, performing better than commercially available systems. Differential proteins were successfully identified in all cases, and these were clearly related to the disease biology. In conclusion, biomarker discovery strategies based on proteomic investigations can be successfully performed on fixed archival tissues by means of this optimized extraction protocol.Summary: Fixed tissue repositories are an ideal information mine for biomarker discovery, with their vast collections of diseased tissues and associated clinical records describing diagnosis, prognosis, therapy, and outcome. Report abuse »
Creative Commons

Related Posters


Methods in Lipidomics Research
Creative Proteomics

Lipidomics Bioinformatics Analysis
Creative Proteomics

Explore Exosome Proteomics at Creative Proteomics
Creative Proteomics

Real-time search of Ubiquitin diGLY modified peptides and PaSER acquisition control on the timsTOF PRO
Christopher Adams1; Robin Park1; Nagarjuna Nagaraj4; Sven Brehmer4; Matt Willetts3; Elizabeth Gordon3; Barry M. Zee2; Hayley Peckham2; Kimberly Lee2; Tharan Srikumar3

Proteomic profiling of IDH mutant gliomas enables prediction of chromosomal copy number variations
Marius Felix 1 2 Dennis Friedel 1 2 Ashok Kumar Jayavelu 4 Uwe Warnken 3 Damian Stichel 1 2 Christel Herold Mende 5 Laura Heikaus 6 Andreas von Deimling 1 2 and David E Reuss 1 2