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Development of low Cost High Throughput miRNA Screening
EP20321
Poster Title: Development of low Cost High Throughput miRNA Screening
Submitted on 19 Dec 2013
Author(s): JF Laes, P Lienard, C Sequaris, I Gonze and JP Detiffe
Affiliations: DNAVision
Poster Views: 1,275
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Poster Information
Abstract: We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA. More than 150 human and more than 100 mouse miRNAs have been validated using as little as 100ng total RNA per miRNA. This method provides accurate sensitive miRNA expression profiles which can be used to identify specific miRNA signatures associated with tissues, biological processes or diseases.
Summary: Micro-RNAs (miRNAs) form a class of 17-24 base singles trandnon-coding RNAs which regulate gene expression post-transcriptionally in eukaryotes and viruses. We have developed a low cost high throughput method to quantify the expression of these miRNAs via classictotal RNA extraction without interference from genomic DNA.Report abuse »
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